Activating Compound | Comment | Organism | Structure |
---|---|---|---|
5'-AMP | increases product formation 10fold by preventing degradation of 3'-phosphoadenylylsulfate | Rattus norvegicus | |
CHAPS | activation, 0.5% w/v | Rattus norvegicus | |
Lubrol Px | activation | Homo sapiens | |
Lubrol Px | activation | Rattus norvegicus | |
Lubrol Px | 3fold stimulation at 0.1% w/v, weak inhibition above 0.1% w/v | Homo sapiens | |
Lubrol Px | 3fold stimulation at 0.1% w/v, weak inhibition above 0.1% w/v | Rattus norvegicus | |
additional information | no activation by octyl glucoside | Rattus norvegicus | |
Nonidet P-40 | activation, 0.5% w/v | Rattus norvegicus | |
Triton X-100 | activation | Homo sapiens | |
Triton X-100 | activation | Rattus norvegicus | |
Tween 20 | activation, 0.5% w/v | Rattus norvegicus | |
Tween 80 | activation, 0.5% w/v | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Lubrol Px | 3fold stimulation at 0.1% w/v, weak inhibition above 0.1% w/v | Rattus norvegicus | |
Mn2+ | above 30 mM, activation below | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi membrane | - |
Mus musculus | 139 | - |
Golgi membrane | - |
Homo sapiens | 139 | - |
Golgi membrane | - |
Rattus norvegicus | 139 | - |
Golgi membrane | - |
Oryctolagus cuniculus | 139 | - |
Golgi membrane | - |
Mesocricetus auratus | 139 | - |
membrane | - |
Mus musculus | 16020 | - |
membrane | - |
Homo sapiens | 16020 | - |
membrane | - |
Rattus norvegicus | 16020 | - |
membrane | - |
Oryctolagus cuniculus | 16020 | - |
membrane | - |
Mesocricetus auratus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 25% of the stimulation with Mn2+, 20 mM | Rattus norvegicus | |
Mn2+ | requirement | Homo sapiens | |
Mn2+ | requirement | Rattus norvegicus | |
Mn2+ | 20 mM | Rattus norvegicus | |
Mn2+ | inhibits above 30 mM | Rattus norvegicus | |
additional information | no requirement of Mg2+ | Rattus norvegicus | |
additional information | no activation by Ca2+, Cd2+, Cu2+ or Zn2+ | Rattus norvegicus | |
NaF | increases product formation 10fold by preventing degradation of 3'-phosphoadenylylsulfate | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + protein tyrosine | Mus musculus | post-translational modification of biologically active peptides and proteins | adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate | - |
? | |
3'-phosphoadenylylsulfate + protein tyrosine | Homo sapiens | post-translational modification of biologically active peptides and proteins | adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate | - |
? | |
3'-phosphoadenylylsulfate + protein tyrosine | Rattus norvegicus | post-translational modification of biologically active peptides and proteins | adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate | - |
? | |
3'-phosphoadenylylsulfate + protein tyrosine | Oryctolagus cuniculus | post-translational modification of biologically active peptides and proteins | adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate | - |
? | |
3'-phosphoadenylylsulfate + protein tyrosine | Mesocricetus auratus | post-translational modification of biologically active peptides and proteins | adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mesocricetus auratus | - |
- |
- |
Mus musculus | - |
- |
- |
Oryctolagus cuniculus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
liver | Rattus norvegicus |
partial | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
cerebellum | - |
Rattus norvegicus | - |
liver | - |
Mus musculus | - |
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Oryctolagus cuniculus | - |
liver | - |
Mesocricetus auratus | - |
lung | - |
Rattus norvegicus | - |
additional information | tissue distribution | Rattus norvegicus | - |
pituitary gland | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
-80°C, in 25% glycerol, 30% loss of activity within 1 month | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + acidic amino acid polymer EAY | i.e. poly-EAY or Glu6,Ala3,Tyr1, model substrate | Mus musculus | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + acidic amino acid polymer EAY | i.e. poly-EAY or Glu6,Ala3,Tyr1, model substrate | Homo sapiens | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + acidic amino acid polymer EAY | i.e. poly-EAY or Glu6,Ala3,Tyr1, model substrate | Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + acidic amino acid polymer EAY | i.e. poly-EAY or Glu6,Ala3,Tyr1, model substrate | Oryctolagus cuniculus | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + acidic amino acid polymer EAY | i.e. poly-EAY or Glu6,Ala3,Tyr1, model substrate | Mesocricetus auratus | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + protein tyrosine | post-translational modification of biologically active peptides and proteins | Mus musculus | adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate | - |
? | |
3'-phosphoadenylylsulfate + protein tyrosine | post-translational modification of biologically active peptides and proteins | Homo sapiens | adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate | - |
? | |
3'-phosphoadenylylsulfate + protein tyrosine | post-translational modification of biologically active peptides and proteins | Rattus norvegicus | adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate | - |
? | |
3'-phosphoadenylylsulfate + protein tyrosine | post-translational modification of biologically active peptides and proteins | Oryctolagus cuniculus | adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate | - |
? | |
3'-phosphoadenylylsulfate + protein tyrosine | post-translational modification of biologically active peptides and proteins | Mesocricetus auratus | adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mus musculus |
30 | - |
assay at | Homo sapiens |
30 | - |
assay at | Rattus norvegicus |
30 | - |
assay at | Oryctolagus cuniculus |
30 | - |
assay at | Mesocricetus auratus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | - |
liver | Rattus norvegicus |
6.7 | - |
sulfation of acidic amino acid polymer EAY | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 7.2 | sulfation of acidic amino acid polymer EAY, liver, about half-maximal activity at pH 6.2 and 7.2 | Rattus norvegicus |