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Literature summary for 2.8.2.20 extracted from

  • Vargas, F.; Schwartz, J.C.
    Apparent identity of cerebral tyrosylsulfotransferase activities using either a cholecystokinin derivative or an acidic amino acid polymer as substrate (1987), FEBS Lett., 211, 234-238.
    View publication on PubMed
Search for more literature for 2.8.2.20

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 requirement Rattus norvegicus
Triton X-100 sulfation of poly-EAY, but not of tert-butoxycarbonylcholecystokinin Rattus norvegicus
Triton X-100 microsomal membrane-bound enzyme, not solubilized enzyme Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
2,6-Dichloro-4-nitrophenol excess concentrations, solubilized enzyme Rattus norvegicus
Acidic amino acid polymer excess concentrations Rattus norvegicus
NaCl above 0.2 M, solubilized enzyme Rattus norvegicus
Poly-EAY substrate inhibition, above 0.01 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
 EAY acidic amino acid polymer, pH 6.6 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Rattus norvegicus 16020
-
microsome
-
 Rattus norvegicus
-
-
additional information subcellular distribution Rattus norvegicus
-
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-40°C, in 10 mM Tris-HCl, pH 7.4, 25% glycerol, 5 mM mercaptoethanol, 1% Triton X-100, 3 days Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + EAY
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + EAY(O-sulfate)
-
 ?
3'-phosphoadenylylsulfate + acidic amino acid polymer EAY i.e. poly-EAY or Glu6,Ala3,Tyr1, model substrate Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4 6.6 solubilized enzyme Rattus norvegicus