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Literature summary for 2.8.2.20 extracted from

  • Lee, R.W.H.; Huttner, W.B.
    Tyrosine-O-sulfated proteins of PC12 pheochromocytoma cells and their sulfation by a tyrosylprotein sulfotransferase (1983), J. Biol. Chem., 258, 11326-11334.
    View publication on PubMed
Search for more literature for 2.8.2.20

General Stability

General Stability Organism
stable in cell lysates prepared by freeze-thawing in 100 mM HEPES-NaOH buffer, pH 7.4, 20 mM MgCl2, 10 mM mercaptoethanol or in 5 mM EDTA or 0.32 M sucrose or by hypoosmotic lysis using 5 mM EDTA, no activity in cell lysates prepared in the presence of Triton X-100 or by sonication Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
EDTA 5 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0083
-
 3'-phosphoadenylylsulfate
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Rattus norvegicus 16020
-
additional information subcellular distribution Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
divalent cations requirement Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylylsulfate + protein tyrosine Rattus norvegicus involved in post-translational processing of specific PC12 cell proteins adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
PC-12 cell fast-responding clone Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + acidic polypeptide tyrosine polypeptides from PC12 cells, referred to as p113, p105, p86 and p84, according to their average MW Rattus norvegicus adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
 ?
3'-phosphoadenylylsulfate + acidic polypeptide tyrosine specifically sulfates Tyr-residues adjacent to acidic amino acids Rattus norvegicus adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
 ?
3'-phosphoadenylylsulfate + protein tyrosine involved in post-translational processing of specific PC12 cell proteins Rattus norvegicus adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus