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Literature summary for 2.8.2.2 extracted from

  • Kohjitani, A.; Fuda, H.; Hanyu, O.; Strott, C.A.
    Cloning, characterization and tissue expression of rat SULT2B1a and SULT2B1b steroid/sterol sulfotransferase isoforms: Divergence of the rat SULT2B1 gene structure from orthologous human and mouse genes (2005), Gene, 367, 66-73.
    View publication on PubMed
Search for more literature for 2.8.2.2

Cloned(Commentary)

Cloned (Comment) Organism
-
 Rattus norvegicus
gene SULT2B1, isozymes SULT2B1a and SULT2B1b as a result of an alternative exon I, DNA and amino acid sequence determination and analysis, genetic structure, expression analysis, comparison of sequence and genetic structure, overview, overexpression of GST-tagged isozymes in Escherichia coli strain BL21(DE3) Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetic analysis of recombinant isozymes SULT2B1a and SULT2B1b Rattus norvegicus
0.0017
-
 cholesterol pH 7.5, 37°C, recombinant isozyme SULT2B1b Rattus norvegicus
0.0089
-
 pregnenolone pH 7.5, 37°C, recombinant isozyme SULT2B1a Rattus norvegicus
0.0179
-
 dehydroepiandrosterone pH 7.5, 37°C, recombinant isozyme SULT2B1a Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38319
-
 x * 42178, SULT2B1a, sequence calculation, x * 38319, SULT2B1b, sequence calculation Rattus norvegicus
42178
-
 x * 42178, SULT2B1a, sequence calculation, x * 38319, SULT2B1b, sequence calculation Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylyl sulfate + cholesterol Rattus norvegicus
-
 adenosine 3',5'-bisphosphate + cholesteryl 3-sulfate
-
 ?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone Rattus norvegicus
-
 adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 ?
3'-phosphoadenylyl sulfate + pregnenolone Rattus norvegicus
-
 adenosine 3',5'-bisphosphate + pregnenolone 3-sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus Q29YR5 isoform SULT2B1a
-
Rattus norvegicus Q29YR5 isoform SULT2B1b
-
Rattus norvegicus Q29YR5 SULT2B1; Sprague-Dawley rats, gene SULT2B1, isozymes SULT2B1a and SULT2B1b as a result of an alternative exon I
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus
recombinant GST-tagged isozymes from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain isoform SULT2B1a Rattus norvegicus
-
brain SULT2B1a is only expressed in brain and testis Rattus norvegicus
-
intestine isoform SULT2B1b Rattus norvegicus
-
intestine main expression of SULT2B1b Rattus norvegicus
-
kidney isoform SULT2B1b Rattus norvegicus
-
kidney main expression of SULT2B1b Rattus norvegicus
-
additional information tissue distribution of isozymes SULT2B1a and SULT2B1b expression, overview Rattus norvegicus
-
skin isoform SULT2B1b Rattus norvegicus
-
skin main expression of SULT2B1b Rattus norvegicus
-
testis isoform SULT2B1a Rattus norvegicus
-
testis SULT2B1a is only expressed in brain and testis Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + cholesterol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + cholesteryl 3-sulfate
-
 ?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 ?
3'-phosphoadenylyl sulfate + pregnenolone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + pregnenolone 3-sulfate
-
 ?
3'-phosphoadenylylsulfate + cholesterol activity is higher than with pregnenolone, isoform SULT2B1b Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + cholesterol poor activity, isoform SULT2B1a Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + pregnenolone isoform SULT2B1a Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + pregnenolone isoform SULT2B1b Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
additional information no activity with dehydroepiandrosterone, isoform SULT2b1a Rattus norvegicus ?
-
 ?
additional information no activity with dehydroepiandrosterone, isoform SULT2b1b Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 42178, SULT2B1a, sequence calculation, x * 38319, SULT2B1b, sequence calculation Rattus norvegicus

Synonyms

Synonyms Comment Organism
steroid/sterol sulfotransferase
-
 Rattus norvegicus
SULT2B1
-
 Rattus norvegicus
SULT2B1a
-
 Rattus norvegicus
SULT2B1b
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Rattus norvegicus

pI Value

Organism Comment pI Value Maximum pI Value
Rattus norvegicus sequence calculation, SULT2B1b
-
 5.12
Rattus norvegicus sequence calculation, SULT2B1a
-
 5.93