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Literature summary for 2.8.2.2 extracted from

  • Takehara, K.; Kubushiro, K.; Iwamori, Y.; Tsukazaki, K.; Nozawa, S.; Iwamori, M.
    Expression of an isoform of testis-specific estrogen sulfotransferase in murine placenta during late gestational period (2001), Arch. Biochem. Biophys., 394, 201-208.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cDNA of placental EST cloned, COS-7 cells transfected with mpEST Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus O35400 mouse
-

Source Tissue

Source Tissue Comment Organism Textmining
epididymis
-
Mus musculus
-
placenta
-
Mus musculus
-
testis testis-specific isoform Mus musculus
-
uterus
-
Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00002965
-
substrate 4-methylumbelliferone Mus musculus
0.0000596
-
substrate p-nitrophenol Mus musculus
0.000207
-
substrate p-hydroxybenzoic acid methyl ester Mus musculus
0.0002474
-
substrate p-nitrocatechol Mus musculus
0.0002614
-
substrate estriol Mus musculus
0.0004035
-
substrate ethynylestradiol Mus musculus
0.0004193
-
substrate estradiol Mus musculus
0.0004737
-
substrate estrone Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + 4-methylumbelliferone
-
Mus musculus adenosine 3',5'-bisphosphate + 4-methylumbelliferone sulfate
-
r
3'-phosphoadenylylsulfate + estriol
-
Mus musculus adenosine 3',5'-bisphosphate + estriol sulfate
-
r
3'-phosphoadenylylsulfate + estrone
-
Mus musculus adenosine 3',5'-bisphosphate + estrone sulfate
-
r
3'-phosphoadenylylsulfate + ethynylestradiol
-
Mus musculus adenosine 3',5'-bisphosphate + ethynylestradiol sulfate
-
r
3'-phosphoadenylylsulfate + methyl 4-hydroxybenzoate
-
Mus musculus adenosine 3',5'-bisphosphate + methyl 4-(sulfooxy)benzoate
-
r
3'-phosphoadenylylsulfate + p-nitrocatechol
-
Mus musculus adenosine 3',5'-bisphosphate + p-nitrocatechol sulfate
-
r
3'-phosphoadenylylsulfate + p-nitrophenol
-
Mus musculus adenosine 3',5'-bisphosphate + p-nitrophenyl sulfate
-
r
additional information exhibits sulfotransferase activity with phenolic hydroxy groups of steroids and artificial substrates, best acceptor substrate is estrogen Mus musculus ?
-
?

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
Mus musculus