Cloned (Comment) | Organism |
---|---|
gene C6st, expression of wild-type and mutant FLAG-tagged C6ST-1s in COS-7 cells, addition of tunicamycin completely abolishes N-glycosylation of the recombinant enzyme and enzyme activity, overview | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
N250S | site-directed mutagenesis, mutation of a putative N-glycosylation site, deletion of the fourth N-glycosylation site from the N-terminus of the enzyme inhibits production of the active C6ST-1, the mutant remains in the endoplasmic reticulum, while all other wild-type and mutant enzymes are located in the Golgi apparatus | Mus musculus |
N413S | site-directed mutagenesis, mutation of a putative N-glycosylation site, deletion of the fourth or sixth N-glycosylation site from the N-terminus of the enzyme inhibits production of the active C6ST-1 | Mus musculus |
N457S | site-directed mutagenesis, mutation of a putative N-glycosylation site, deletion of the fifth N-glycosylation site from the N-terminus of the enzyme results in a marked loss of the keratan sulfate sulfotransferase activity | Mus musculus |
N63S | site-directed mutagenesis, mutation of a putative N-glycosylation site | Mus musculus |
N74S | site-directed mutagenesis, mutation of a putative N-glycosylation site | Mus musculus |
N96S | site-directed mutagenesis, mutation of a putative N-glycosylation site | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
PNGase F | deglycosylation inactivates the enzyme | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00034 | - |
3'-phosphoadenylyl sulfate | pH 6.8, 37°C, recombinant mutant N250S, with chondroitin | Mus musculus | |
0.0004 | - |
3'-phosphoadenylyl sulfate | pH 6.8, 37°C, recombinant mutant N457S, with chondroitin | Mus musculus | |
0.00076 | - |
chondroitin | pH 6.8, 37°C, recombinant mutant N250S | Mus musculus | |
0.00103 | - |
chondroitin | pH 6.8, 37°C, recombinant mutant N457S | Mus musculus | |
0.0011 | - |
3'-phosphoadenylyl sulfate | pH 6.8, 37°C, recombinant wild-type enzyme, with chondroitin | Mus musculus | |
0.00144 | - |
chondroitin | pH 6.8, 37°C, recombinant mutant N413S | Mus musculus | |
0.00146 | - |
chondroitin | pH 6.8, 37°C, recombinant wild-type enzyme | Mus musculus | |
0.0029 | - |
Keratan sulfate | pH 6.8, 37°C, recombinant mutant N413S | Mus musculus | |
0.0029 | - |
3'-phosphoadenylyl sulfate | pH 6.8, 37°C, recombinant mutant N413S, with keratan sulfate | Mus musculus | |
0.0038 | - |
3'-phosphoadenylyl sulfate | pH 6.8, 37°C, recombinant wild-type enzyme, with keratan sulfate | Mus musculus | |
0.00417 | - |
3'-phosphoadenylyl sulfate | pH 6.8, 37°C, recombinant mutant N413S, with chondroitin | Mus musculus | |
0.005 | - |
Keratan sulfate | pH 6.8, 37°C, recombinant wild-type enzyme | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Mus musculus | 5794 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
x * 67000, glycosylated recombinant enzyme, SDS-PAGE, x * 54000, deglycosylated recombinant enzyme, SDS-PAGE | Mus musculus |
67000 | - |
x * 67000, glycosylated recombinant enzyme, SDS-PAGE, x * 54000, deglycosylated recombinant enzyme, SDS-PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + chondroitin | Mus musculus | - |
adenosine 3',5'-bisphosphate + chondroitin 6-sulfate | - |
? | |
3'-phosphoadenylyl sulfate + keratan sulfate | Mus musculus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | O88199 | gene Chst3, C6st, or Gst0; isozyme C6ST-1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme contains N-linked oligosaccharide side chains, which have essential roles in the generation of the active enzyme and in its keratan sulfate sulfotransferase activity, overview, six potential N-glycosylation sites | Mus musculus |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant FLAG-tagged C6ST-1s from COS-7 cells by anti-FLAG-tag immunoaffinity and wheat germ agglutinin affinity chromatography | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + chondroitin | - |
Mus musculus | adenosine 3',5'-bisphosphate + chondroitin 6-sulfate | - |
? | |
3'-phosphoadenylyl sulfate + keratan sulfate | - |
Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 67000, glycosylated recombinant enzyme, SDS-PAGE, x * 54000, deglycosylated recombinant enzyme, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
C6ST-1 | - |
Mus musculus |
chondroitin 6-sulfotransferase-1 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
keratan sulfate sulfotransferase activity, assay at | Mus musculus |
6.8 | - |
chondroitin sulfotransferase activity, assay at | Mus musculus |