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Literature summary for 2.8.2.17 extracted from

  • Hollmann, J.; Niemann, R.; Buddecke, E.
    Purification and characterization of a 3-phosphoadenylylsulfate:chondroitin 6-sulfotransferase from arterial tissue (1986), Biol. Chem. Hoppe-Seyler, 367, 5-13.
    View publication on PubMed
Search for more literature for 2.8.2.17

Activating Compound

Activating Compound Comment Organism Structure
Chondroitin sulfates competitive inhibitors Bos taurus
lysozyme stimulates 2fold at 1.5 mg/ml Bos taurus
poly-L-lysine stimulates 2fold at 1.5 mg/ml Bos taurus
protamine stimulates 2fold at 1.5 mg/ml Bos taurus
spermidine stimulates 2fold at 1 mM Bos taurus
spermine stimulates 2fold at 1 mM Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 chondroitin
-
 Bos taurus
0.48
-
 dodecasaccharide derived from chondroitin Bos taurus
0.9
-
 decasaccharide derived from chondroitin Bos taurus
2.5
-
 Octasaccharide derived from chondroitin Bos taurus
5
-
 hexasaccharide derived from chondroitin Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Bos taurus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ divalent cation required, Mn2+, Mg2+, Ca2+ Bos taurus
Mg2+ divalent cation required, Mn2+, Mg2+, Ca2+ Bos taurus
Mn2+ divalent cation required, Mn2+, Mg2+, Ca2+ Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 x * 38000, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylylsulfate + chondroitin Bos taurus is believed to act in the course of chondroitin sulfate synthesis in cooperation with, but shortly after the enzymes involved in the chain elongation reaction adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 calf
-

Purification (Commentary)

Purification (Comment) Organism
calf Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
artery
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + decasaccharide
-
 Bos taurus ?
-
 r
3'-phosphoadenylyl sulfate + dodecasaccharide
-
 Bos taurus ?
-
 r
3'-phosphoadenylyl sulfate + hexasaccharide
-
 Bos taurus ?
-
 r
3'-phosphoadenylyl sulfate + octasaccharide
-
 Bos taurus ?
-
 r
3'-phosphoadenylylsulfate + chondroitin specificity towards desulfated chondroitin sulfate and oligosaccharides derived therefrom Bos taurus adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
-
 ?
3'-phosphoadenylylsulfate + chondroitin is believed to act in the course of chondroitin sulfate synthesis in cooperation with, but shortly after the enzymes involved in the chain elongation reaction Bos taurus adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
-
 ?
3'-phosphoadenylylsulfate + chondroitin-derived oligosaccharides even-numbered oligosaccharides with a glucuronic acid at the nonreducing terminus are active as acceptors, their capacity decreasing with decreasing chain length: dodecasaccharide at 64%, decasaccharide at 55%, octasaccharide at 17%, hexasaccharide at 4% of the chondroitin sulfation Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 double pH-optimum: 100% at pH 6.0 and 65% at pH 7.3 Bos taurus
7.3
-
 double pH-optimum: 100% at pH 6.0 and 65% at pH 7.3 Bos taurus