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Literature summary for 2.8.2.1 extracted from

  • Hattori, K.; Motohashi, N.; Kobayashi, I.; Tohya, T.; Oikawa, M.; Tamura, H.O.
    Cloning, expression, and characterization of cytosolic sulfotransferase isozymes from Drosophila melanogaster (2008), Biosci. Biotechnol. Biochem., 72, 540-547.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
isoforms ST1, ST3, and ST4 are expressed in Escherichia coli BL21(DE3) cells Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
3'-phosphoadenylyl sulfate isoform ST4, in 50 mM sodium phosphate, pH 6.8, at 37°C Drosophila melanogaster
0.0021
-
3'-phosphoadenylyl sulfate isoform ST1, in 50 mM sodium phosphate, pH 6.8, at 37°C Drosophila melanogaster
0.024
-
3'-phosphoadenylyl sulfate isoform ST3, in 50 mM sodium phosphate, pH 6.8, at 37°C Drosophila melanogaster
0.037
-
4-nitrophenol isoform ST4, in 50 mM sodium phosphate, pH 6.8, at 37°C Drosophila melanogaster
0.24
-
4-nitrophenol isoform ST1, in 50 mM sodium phosphate, pH 6.8, at 37°C Drosophila melanogaster
1.3
-
4-nitrophenol isoform ST3, in 50 mM sodium phosphate, pH 6.8, at 37°C Drosophila melanogaster

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ MgCl2 (10 mM) enhances isoform ST3 activity by 50%, whereas no effects of these cations are observed for isoforms ST1 and ST4 Drosophila melanogaster

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
isoform ST2, estimated from amino acid sequence Drosophila melanogaster
37000
-
isoform ST3, estimated from amino acid sequence Drosophila melanogaster
39000
-
isoform ST1, estimated from amino acid sequence Drosophila melanogaster
40000
-
isoform ST1, gel filtration Drosophila melanogaster
40000
-
isoform ST3, gel filtration Drosophila melanogaster
41000
-
isoform ST4, estimated from amino acid sequence Drosophila melanogaster
52000
-
isoform ST4, gel filtration Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylyl sulfate + 1-naphthol Drosophila melanogaster isoform ST4 shows a preference toward 1-naphtol adenosine 3',5'-bisphosphate + 1-naphthyl sulfate
-
?
3'-phosphoadenylyl sulfate + 4-nitrophenol Drosophila melanogaster isoform ST3 shows higher sulfating activity toward 4-nitrophenol adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
?
3'-phosphoadenylyl sulfate + bisphenol A Drosophila melanogaster isoform ST3 also shows sulfating activity toward bisphenol A adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylyl sulfate + dopamine Drosophila melanogaster isoform ST1 recognizes dopamine as a substrate adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylyl sulfate + vanillin Drosophila melanogaster vanillin is the best substrate for isoforms ST1 and ST3 adenosine 3',5'-bisphosphate + ?
-
?
additional information Drosophila melanogaster isoform ST4 shows low but not negligible activity toward 20-hydroxyecdysone ?
-
?
additional information Drosophila melanogaster no activity toward tyramine and serotonin ?
-
?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose column chromatography and glutathione Sepharose gel filtration Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + 1-naphthol isoform ST4 shows a preference toward 1-naphtol Drosophila melanogaster adenosine 3',5'-bisphosphate + 1-naphthyl sulfate
-
?
3'-phosphoadenylyl sulfate + 4-nitrophenol isoform ST3 shows higher sulfating activity toward 4-nitrophenol Drosophila melanogaster adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
?
3'-phosphoadenylyl sulfate + bisphenol A isoform ST3 also shows sulfating activity toward bisphenol A Drosophila melanogaster adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylyl sulfate + dopamine isoform ST1 recognizes dopamine as a substrate Drosophila melanogaster adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylyl sulfate + vanillin vanillin is the best substrate for isoforms ST1 and ST3 Drosophila melanogaster adenosine 3',5'-bisphosphate + ?
-
?
additional information isoform ST4 shows low but not negligible activity toward 20-hydroxyecdysone Drosophila melanogaster ?
-
?
additional information no activity toward tyramine and serotonin Drosophila melanogaster ?
-
?

Synonyms

Synonyms Comment Organism
cytosolic sulfotransferase
-
Drosophila melanogaster
ST1 isoform Drosophila melanogaster
ST2 isoform Drosophila melanogaster
ST3 isoform Drosophila melanogaster
ST4 isoform Drosophila melanogaster
SULT
-
Drosophila melanogaster

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
isoform ST4 Drosophila melanogaster
35
-
isoform ST1 Drosophila melanogaster

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.5
-
3'-phosphoadenylyl sulfate isoform ST1, in 50 mM sodium phosphate, pH 6.8, at 37°C Drosophila melanogaster
27
-
3'-phosphoadenylyl sulfate isoform ST4, in 50 mM sodium phosphate, pH 6.8, at 37°C Drosophila melanogaster
30
-
4-nitrophenol isoform ST1, in 50 mM sodium phosphate, pH 6.8, at 37°C Drosophila melanogaster
40.7
-
3'-phosphoadenylyl sulfate isoform ST3, in 50 mM sodium phosphate, pH 6.8, at 37°C Drosophila melanogaster
70
-
4-nitrophenol isoform ST4, in 50 mM sodium phosphate, pH 6.8, at 37°C Drosophila melanogaster
330
-
4-nitrophenol isoform ST3, in 50 mM sodium phosphate, pH 6.8, at 37°C Drosophila melanogaster

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4 6.8 isoform ST3 Drosophila melanogaster

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
pH has little effect on the activities of isoforms ST1 and ST4 Drosophila melanogaster