Cloned (Comment) | Organism |
---|---|
isoforms ST1, ST3, and ST4 are expressed in Escherichia coli BL21(DE3) cells | Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
3'-phosphoadenylyl sulfate | isoform ST4, in 50 mM sodium phosphate, pH 6.8, at 37°C | Drosophila melanogaster | |
0.0021 | - |
3'-phosphoadenylyl sulfate | isoform ST1, in 50 mM sodium phosphate, pH 6.8, at 37°C | Drosophila melanogaster | |
0.024 | - |
3'-phosphoadenylyl sulfate | isoform ST3, in 50 mM sodium phosphate, pH 6.8, at 37°C | Drosophila melanogaster | |
0.037 | - |
4-nitrophenol | isoform ST4, in 50 mM sodium phosphate, pH 6.8, at 37°C | Drosophila melanogaster | |
0.24 | - |
4-nitrophenol | isoform ST1, in 50 mM sodium phosphate, pH 6.8, at 37°C | Drosophila melanogaster | |
1.3 | - |
4-nitrophenol | isoform ST3, in 50 mM sodium phosphate, pH 6.8, at 37°C | Drosophila melanogaster |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | MgCl2 (10 mM) enhances isoform ST3 activity by 50%, whereas no effects of these cations are observed for isoforms ST1 and ST4 | Drosophila melanogaster |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
isoform ST2, estimated from amino acid sequence | Drosophila melanogaster |
37000 | - |
isoform ST3, estimated from amino acid sequence | Drosophila melanogaster |
39000 | - |
isoform ST1, estimated from amino acid sequence | Drosophila melanogaster |
40000 | - |
isoform ST1, gel filtration | Drosophila melanogaster |
40000 | - |
isoform ST3, gel filtration | Drosophila melanogaster |
41000 | - |
isoform ST4, estimated from amino acid sequence | Drosophila melanogaster |
52000 | - |
isoform ST4, gel filtration | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + 1-naphthol | Drosophila melanogaster | isoform ST4 shows a preference toward 1-naphtol | adenosine 3',5'-bisphosphate + 1-naphthyl sulfate | - |
? | |
3'-phosphoadenylyl sulfate + 4-nitrophenol | Drosophila melanogaster | isoform ST3 shows higher sulfating activity toward 4-nitrophenol | adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate | - |
? | |
3'-phosphoadenylyl sulfate + bisphenol A | Drosophila melanogaster | isoform ST3 also shows sulfating activity toward bisphenol A | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylyl sulfate + dopamine | Drosophila melanogaster | isoform ST1 recognizes dopamine as a substrate | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylyl sulfate + vanillin | Drosophila melanogaster | vanillin is the best substrate for isoforms ST1 and ST3 | adenosine 3',5'-bisphosphate + ? | - |
? | |
additional information | Drosophila melanogaster | isoform ST4 shows low but not negligible activity toward 20-hydroxyecdysone | ? | - |
? | |
additional information | Drosophila melanogaster | no activity toward tyramine and serotonin | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione Sepharose column chromatography and glutathione Sepharose gel filtration | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + 1-naphthol | isoform ST4 shows a preference toward 1-naphtol | Drosophila melanogaster | adenosine 3',5'-bisphosphate + 1-naphthyl sulfate | - |
? | |
3'-phosphoadenylyl sulfate + 4-nitrophenol | isoform ST3 shows higher sulfating activity toward 4-nitrophenol | Drosophila melanogaster | adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate | - |
? | |
3'-phosphoadenylyl sulfate + bisphenol A | isoform ST3 also shows sulfating activity toward bisphenol A | Drosophila melanogaster | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylyl sulfate + dopamine | isoform ST1 recognizes dopamine as a substrate | Drosophila melanogaster | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylyl sulfate + vanillin | vanillin is the best substrate for isoforms ST1 and ST3 | Drosophila melanogaster | adenosine 3',5'-bisphosphate + ? | - |
? | |
additional information | isoform ST4 shows low but not negligible activity toward 20-hydroxyecdysone | Drosophila melanogaster | ? | - |
? | |
additional information | no activity toward tyramine and serotonin | Drosophila melanogaster | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cytosolic sulfotransferase | - |
Drosophila melanogaster |
ST1 | isoform | Drosophila melanogaster |
ST2 | isoform | Drosophila melanogaster |
ST3 | isoform | Drosophila melanogaster |
ST4 | isoform | Drosophila melanogaster |
SULT | - |
Drosophila melanogaster |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
isoform ST4 | Drosophila melanogaster |
35 | - |
isoform ST1 | Drosophila melanogaster |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.5 | - |
3'-phosphoadenylyl sulfate | isoform ST1, in 50 mM sodium phosphate, pH 6.8, at 37°C | Drosophila melanogaster | |
27 | - |
3'-phosphoadenylyl sulfate | isoform ST4, in 50 mM sodium phosphate, pH 6.8, at 37°C | Drosophila melanogaster | |
30 | - |
4-nitrophenol | isoform ST1, in 50 mM sodium phosphate, pH 6.8, at 37°C | Drosophila melanogaster | |
40.7 | - |
3'-phosphoadenylyl sulfate | isoform ST3, in 50 mM sodium phosphate, pH 6.8, at 37°C | Drosophila melanogaster | |
70 | - |
4-nitrophenol | isoform ST4, in 50 mM sodium phosphate, pH 6.8, at 37°C | Drosophila melanogaster | |
330 | - |
4-nitrophenol | isoform ST3, in 50 mM sodium phosphate, pH 6.8, at 37°C | Drosophila melanogaster |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | 6.8 | isoform ST3 | Drosophila melanogaster |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH has little effect on the activities of isoforms ST1 and ST4 | Drosophila melanogaster |