Literature summary for 2.8.2.1 extracted from

Allali-Hassani, A.; Pan, P.W.; Dombrovski, L.; Najmanovich, R.; Tempel, W.; Dong, A.; Loppnau, P.; Martin, F.; Thonton, J.; Edwards, A.M.; Bochkarev, A.; Plotnikov, A.N.; Vedadi, M.; Arrowsmith, C.H.
Structural and chemical profiling of the human cytosolic sulfotransferases (2007), PLoS Biol., 5, 1063-1078.

No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified SULT1B1, SULT1C1, and SULT1C3 are crystallized in the presence of 2 mM PAP using the hanging drop method at 20°C by mixing for SULT1B1: 0.002 ml of the protein solution with 0.002 ml of the reservoir solution containing 0.1 M Bis-Tris, pH 6.5, 0.2 M ammonium sulfate and 16%-20% PEG 4000, for SULT1C1: 0.002 ml of the protein solution with 0.002 ml of the reservoir solution containing 0.1 M K2HPO4 and 12%-16% PEG 3350, and for SULT1C3: 0.002 ml of the protein solution with 0.002 ml of the reservoir solution containing 18% PEG 3350, 0.2 M ammonium formate, 0.1 M Bis-Tris (pH 6.5), to obtain crystals of SULT1C2-PAP-PCP ternary complex, 10 mg/ml of purified SULT1C2 is mixed with 2 mM PAP and 2 mM PCP in 20 mM MES-NaOH buffer, pH 6.5, and incubated on ice for 30 min, SULT1C2-PAP-PCP complex is crystallized using the sitting drop method at 20°C by mixing 0.0008 ml of the protein-cofactor-inhibitor mix with 0.0008 ml of the reservoir solution containing 25% PEGl 3350, 0.2 M lithium sulfate, 0.1 M Bis-Tris, pH 6.5, SULT4A1 and SULT1C2 crystals are obtained by using the hanging drop method at 20°C by mixing 0.002 ml of the protein solution with 0.002 ml of the reservoir solution containing 20% PEG 4000, 0.2 M ammonium tartrate, and 14%-20% PEG 3350, 0.2 M lithium citrate, 0.1 M sodium citrate, pH 4.6, respectively	Homo sapiens

Crystallization (Comment)

Organism

purified SULT1B1, SULT1C1, and SULT1C3 are crystallized in the presence of 2 mM PAP using the hanging drop method at 20°C by mixing for SULT1B1: 0.002 ml of the protein solution with 0.002 ml of the reservoir solution containing 0.1 M Bis-Tris, pH 6.5, 0.2 M ammonium sulfate and 16%-20% PEG 4000, for SULT1C1: 0.002 ml of the protein solution with 0.002 ml of the reservoir solution containing 0.1 M K2HPO4 and 12%-16% PEG 3350, and for SULT1C3: 0.002 ml of the protein solution with 0.002 ml of the reservoir solution containing 18% PEG 3350, 0.2 M ammonium formate, 0.1 M Bis-Tris (pH 6.5), to obtain crystals of SULT1C2-PAP-PCP ternary complex, 10 mg/ml of purified SULT1C2 is mixed with 2 mM PAP and 2 mM PCP in 20 mM MES-NaOH buffer, pH 6.5, and incubated on ice for 30 min, SULT1C2-PAP-PCP complex is crystallized using the sitting drop method at 20°C by mixing 0.0008 ml of the protein-cofactor-inhibitor mix with 0.0008 ml of the reservoir solution containing 25% PEGl 3350, 0.2 M lithium sulfate, 0.1 M Bis-Tris, pH 6.5, SULT4A1 and SULT1C2 crystals are obtained by using the hanging drop method at 20°C by mixing 0.002 ml of the protein solution with 0.002 ml of the reservoir solution containing 20% PEG 4000, 0.2 M ammonium tartrate, and 14%-20% PEG 3350, 0.2 M lithium citrate, 0.1 M sodium citrate, pH 4.6, respectively

Homo sapiens

Inhibitors

Inhibitors	Comment	Organism
3,5-dibromo-4-hydroxy-benzoic acid (6-chloro-4-oxo-4H-chromen-3-ylmethylene)-hydrazide	DBHM	Homo sapiens
3,5-dibromo-4-hydroxybenzoic acid (6,8-dichloro-4-oxo-4H-chromen-3-ylmethylene) hydrazide	DBHD	Homo sapiens
adenosine 5'-(beta,gamma-imido) triphosphate	i.e. AMP-PNP, a non-hydrolysable ATP analogue	Homo sapiens
additional information	isoprenaline is poor inhibitor of SULT1B1	Homo sapiens
pyridoxal 5'-phosphate	a competitive inhibitor for sulfotransferases	Homo sapiens
quercetin	a potent inhibitor of SULT1A1	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Homo sapiens	5829	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	SULTs play a role in the metabolism of regulatory hormones, drugs, and carcinogens, they catalyze the sulfonation of simple phenols, estradiol, and thyroid hormones, as well as environmental xenobiotics and drugs, overview	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate	random bi-bi mechanism catalytic mechanism of SULTs, many family members have distinct, but overlapping substrate specificities, the enzymes have a sequential catalytic mechanism that is susceptible to substrate inhibition	Homo sapiens	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	SULT4A1, an orphan member of the SULT family expressed primarily in the brain	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
3'-phosphoadenylyl sulfate + 1-naphthol	SULT1B1	Homo sapiens	adenosine 3',5'-bisphosphate + 1-naphthyl sulfate	-	?
additional information	SULTs play a role in the metabolism of regulatory hormones, drugs, and carcinogens, they catalyze the sulfonation of simple phenols, estradiol, and thyroid hormones, as well as environmental xenobiotics and drugs, overview	Homo sapiens	?	-	?
additional information	role for 3'-phosphoadenylyl sulfate in priming the conformation of substrate binding loops, overview, substrate specificities and binding structures of SULT1B1, SULT1A1, SULT1A3, SULT1C1, SULT1C2, and SULT1C3, overview	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
More	detailed structural analysis and comparison of SULT family enzymes, comparison of substrate binding structures of SULT1B1, SULT1A1, SULT1A3, SULT1C1, SULT1C2, and SULT1C3, overview, chemical library preparation and ligand binding screens	Homo sapiens

Synonyms

Synonyms	Comment	Organism
cytosolic sulfotransferase	-	Homo sapiens
SULT	-	Homo sapiens
SULT1A1	-	Homo sapiens
SULT1A2	-	Homo sapiens
SULT1A3	-	Homo sapiens
SULT1B1	-	Homo sapiens
SULT1C1	-	Homo sapiens
SULT1C2	-	Homo sapiens
SULT1C3	-	Homo sapiens
SULT4A1	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Homo sapiens