Cloned (Comment) | Organism |
---|---|
recombinant enzyme form SULT1 ST5 is expressed using the pGEX-2TK bacterial expression system, expression in BL21 (DE3) cells | Danio rerio |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | recombinant enzyme form SULT1 ST5 | Danio rerio | |
Co2+ | recombinant enzyme form SULT1 ST5 | Danio rerio | |
Cu2+ | recombinant enzyme form SULT1 ST5 | Danio rerio | |
Fe2+ | recombinant enzyme form SULT1 ST5 | Danio rerio | |
Hg2+ | recombinant enzyme form SULT1 ST5 | Danio rerio | |
Pb2+ | recombinant enzyme form SULT1 ST5 | Danio rerio | |
Zn2+ | recombinant enzyme form SULT1 ST5 | Danio rerio |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0277 | - |
3,3',5-triiodo-D-thyronine | pH 8.0, 28°C, enzyme form SULT1 ST5 | Danio rerio | |
0.0387 | - |
3,3',5-triiodo-L-thyronine | pH 8.0, 28°C, enzyme form SULT1 ST5 | Danio rerio | |
0.0445 | - |
L-thyroxine | pH 8.0, 28°C, enzyme form SULT1 ST5 | Danio rerio | |
0.0977 | - |
beta-naphthol | pH 8.0, 28°C, enzyme form SULT1 ST5 | Danio rerio |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Danio rerio | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | significant stimulation of enzyme form SULT1 ST5 | Danio rerio |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | - |
recombinant enzyme form SULT1 ST5 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme form SULT1 ST5 expressed in BL21 (DE3) cells | Danio rerio |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | significant level of expression at the beginning of the hatching period during embryogenesis, enzyme form SULT1 ST5 | Danio rerio | - |
larva | gradual increase in expression to a high level of expression throughout the larval stage of maturity, enzyme form SULT1 ST5 | Danio rerio | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + 2-naphthol | recombinant enzyme form SULT1 ST5 | Danio rerio | adenosine 3',5'-bisphosphate + 2-naphthylsulfate | - |
? | |
3'-phosphoadenylylsulfate + 3,3',5-triiodo-D-thyronine | recombinant enzyme form SULT1 ST5 | Danio rerio | adenosine 3',5'-bisphosphate + 3,3',5-triiodo-D-thyronine 4'-sulfate | - |
? | |
3'-phosphoadenylylsulfate + 3,3',5-triiodo-L-thyronine | recombinant enzyme form SULT1 ST5 | Danio rerio | adenosine 3',5'-bisphosphate + 3,3',5-triiodo-L-thyronine 4'-sulfate | - |
? | |
3'-phosphoadenylylsulfate + 4-nitrophenol | recombinant enzyme form SULT1 ST5 | Danio rerio | adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate | - |
? | |
3'-phosphoadenylylsulfate + butylated hydroxyanisole | recombinant enzyme form SULT1 ST5 | Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + caffeic acid | recombinant enzyme form SULT1 ST5 | Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + catechin | recombinant enzyme form SULT1 ST5 | Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + chlorogenic acid | recombinant enzyme form SULT1 ST5 | Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + daidzein | recombinant enzyme form SULT1 ST5 | Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + gallic acid | recombinant enzyme form SULT1 ST5 | Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + genistein | recombinant enzyme form SULT1 ST5 | Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + kaempferol | recombinant enzyme form SULT1 ST5 | Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + L-thyroxine | recombinant enzyme form SULT1 ST5 | Danio rerio | adenosine 3',5'-bisphosphate + L-thyroxine 4'-sulfate | - |
? | |
3'-phosphoadenylylsulfate + myricetin | recombinant enzyme form SULT1 ST5 | Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + n-propyl gallate | recombinant enzyme form SULT1 ST5 | Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + quercetin | recombinant enzyme form SULT1 ST5 | Danio rerio | adenosine 3',5'-bisphosphate + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SULT1 ST5 | - |
Danio rerio |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
with beta-naphthol as substrate, two optima at pH 6.0 and at pH 9.0 with 3,3',5-triiodo-L-thyronine as substrate, enzyme form SULT1 ST5 | Danio rerio |
9 | - |
two optima at pH 6.0 and at pH 9.0 with 3,3',5-triiodo-L-thyronine as substrate, enzyme form SULT1 ST5 | Danio rerio |