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Literature summary for 2.8.2.1 extracted from

  • Chen, G.; Chen, X.
    Arginine residues in the active site of human phenol sulfotransferase (SULT1A1) (2003), J. Biol. Chem., 278, 36358-36364.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of SULT1A1 wild-type and mutants as maltose-binding fusion protein in Escherichia coli XL1-Blue Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information
-
 Homo sapiens
R130A site-directed mutagenesis, SULT1A1 mutant, unaltered activity Homo sapiens
R130E site-directed mutagenesis, SULT1A1 mutant, slightly increased activity Homo sapiens
R213E site-directed mutagenesis, SULT1A1 mutant, unaltered activity Homo sapiens
R257A site-directed mutagenesis, SULT1A1 mutant, no activity Homo sapiens
R257E site-directed mutagenesis, SULT1A1 mutant, no activity Homo sapiens
R78A site-directed mutagenesis, SULT1A1 mutant, unaltered activity Homo sapiens
R78E site-directed mutagenesis, SULT1A1 mutant, no activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2,3-Butanedione Arg-specific modification reagent; SULT1A1, inactivation Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of Arg mutants Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol SULT1A1 Homo sapiens 5829
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P50225 SULT1A1 is a P-PST enzyme form
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme as maltose-binding fusion protein from Escherichia coli Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate structure modeling to determine Arg residues involved in the catalytic reaction: Arg257 is essential, located very close to 3'-phosphoadenylyl sulfate, the negative charge of Arg78 is not required, Arg130 is not critical Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + a phenol
-
 Homo sapiens adenosine 3',5'-bisphosphate + an aryl sulfate
-
 ?
3'-phosphoadenylylsulfate + 2-naphthol SULT1A1 Homo sapiens adenosine 3',5'-bisphosphate + 2-naphthyl sulfate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
 assay at Homo sapiens