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Literature summary for 2.8.2.1 extracted from

  • Dajani, R.; Hood, A.M.; Coughtrie, M.W.H.
    A single amino acid, Glu146, governs the substrate specificity of a human dopamine sulfotransferase, SULT1A3 (1998), Mol. Pharmacol., 54, 942-948.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
SULT1A1, DNA sequence determination and analysis, expression in Escherichia coli Homo sapiens
SULT1A3, wild-type and mutants, expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
E146A site-directed mutagenesis, reduced activity with dopamine and increased activity with 4-nitrophenol compared to wild-type SULT1A3, switch of specificity to wild-type SULT1A1 Homo sapiens
H143Y site-directed mutagenesis, reduced activity with dopamine compared to wild-type SULT1A3 Homo sapiens
H143Y/E146A site-directed mutagenesis, reduced activity with dopamine and increased activity with 4-nitrophenol compared to wild-type SULT1A3, switch of specificity to wild-type SULT1A1 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
3'-phosphoadenylylsulfate SULT1A3 mutant H143Y, pH 7.4, 37°C Homo sapiens
0.0022
-
dopamine wild-type SULT1A3, pH 7.4, 37°C Homo sapiens
0.003
-
3'-phosphoadenylylsulfate SULT1A3 mutant E146A, pH 7.4, 37°C Homo sapiens
0.0038
-
4-nitrophenol pH 7.4, 37°C Homo sapiens
0.0038
-
4-nitrophenol wild-type SULT1A1 Homo sapiens
0.0043
-
3'-phosphoadenylylsulfate wild-type SULT1A1, pH 7.4, 37°C Homo sapiens
0.0046
-
3'-phosphoadenylylsulfate wild-type SULT1A3, pH 7.4, 37°C Homo sapiens
0.0048
-
3'-phosphoadenylylsulfate SULT1A3 double mutant H143Y/E146A, pH 7.4, 37°C Homo sapiens
0.0056
-
dopamine SULT1A3 mutant H143Y, pH 7.4, 37°C Homo sapiens
0.0061
-
4-nitrophenol SULT1A3 mutant E146A, pH 7.4, 37°C Homo sapiens
0.0094
-
4-nitrophenol SULT1A3 double mutant H143Y/E146A, pH 7.4, 37°C Homo sapiens
0.087
-
dopamine SULT1A3 double mutant H143Y/E146A, pH 7.4, 37°C Homo sapiens
0.109
-
dopamine wild-type SULT1A1, pH 7.4, 37°C Homo sapiens
0.127
-
dopamine SULT1A3 mutant E146A, pH 7.4, 37°C Homo sapiens
1.38
-
4-nitrophenol wild-type SULT1A3, pH 7.4, 37°C Homo sapiens
1.47
-
4-nitrophenol SULT1A3 mutant H143Y, pH 7.4, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
SULT1A3, is a M-PST enzyme form
-
Homo sapiens P50225 SULT1A1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from Escherichia coli Homo sapiens
recombinant wild-type SULT1A3 and mutants from Escherichia coli Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate Glu146 determines the substrate specificity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver SULT1A1 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + a phenol
-
Homo sapiens adenosine 3',5'-bisphosphate + an aryl sulfate
-
?
3'-phosphoadenylylsulfate + 4-nitrophenol
-
Homo sapiens adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
?
3'-phosphoadenylylsulfate + dopamine
-
Homo sapiens adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylylsulfate + dopamine SULT1A3 is specific for dopamine and other biogenic amines Homo sapiens adenosine 3',5'-bisphosphate + ?
-
?
additional information substrate specificity Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
SULT
-
Homo sapiens