Literature summary for 2.8.1.B3 extracted from

Naumann, P.T.; Lauhon, C.T.; Ficner, R.
Purification, crystallization and preliminary crystallographic analysis of a 4-thiouridine synthetase-RNA complex (2013), Acta Crystallogr. Sect. F, 69, 421-424.

Search for more literature for 2.8.1.B3

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Thermotoga maritima
expressed in Escherichia coli BL21(DE3)pLysS cells	Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with RNA (truncated tRNAPhe), sitting drop vapor diffusion method, using 2 M sodium formate, 100 mM sodium citrate pH 4.6, 2 mM dithiothreitol, at 20°C	Thermotoga maritima
sitting drop vapor diffusion method, using 2.0 M sodium formate, 100 mM sodium citrate pH 4.6, 2 mM dithiothreitol	Thermotoga maritima

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
44000	-	x * 44000	Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
sulfurtransferase IscS-SSH + adenylated-tRNA-uridine(position8)	Thermotoga maritima	-	sulfurtransferase IscS-SH + tRNA-4-thiouridine(position8) + AMP	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	Q9X220	-	-
Thermotoga maritima	Q9X220	cf. 2.8.1.4	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermotoga maritima
DEAE-Sepharose column chromatography and Superdex 75 gel filtration	Thermotoga maritima

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sulfurtransferase IscS-SSH + adenylated-tRNA-uridine(position8)	-	Thermotoga maritima	sulfurtransferase IscS-SH + tRNA-4-thiouridine(position8) + AMP	-	?

Subunits

Subunits	Comment	Organism
?	x * 44000, SDS-PAGE	Thermotoga maritima
?	x * 44000	Thermotoga maritima

Synonyms

Synonyms	Comment	Organism
4-thiouridine synthetase	-	Thermotoga maritima
ThiI	-	Thermotoga maritima

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Release 2023.1 (January 2023)