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Literature summary for 2.8.1.B2 extracted from

  • Leidel, S.; Pedrioli, P.G.; Bucher, T.; Brost, R.; Costanzo, M.; Schmidt, A.; Aebersold, R.; Boone, C.; Hofmann, K.; Peter, M.
    Ubiquitin-related modifier Urm1 acts as a sulphur carrier in thiolation of eukaryotic transfer RNA (2009), Nature, 458, 228-232.
    View publication on PubMed
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Urm1p-terminal-Gly-AMP + Uba4-SSH Saccharomyces cerevisiae Uba4p first adenylates and then directly transfers sulfur onto Urm1p. Thiolation function of Urm1p is critical to regulate cellular responses to nutrient starvation and oxidative stress conditions, most likely by increasing translation fidelity Urm1p-terminal-Gly-COSH + Uba4-SH + AMP
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Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P38820
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Urm1p-terminal-Gly-AMP + Uba4-SSH Uba4p first adenylates and then directly transfers sulfur onto Urm1p. Thiolation function of Urm1p is critical to regulate cellular responses to nutrient starvation and oxidative stress conditions, most likely by increasing translation fidelity Saccharomyces cerevisiae Urm1p-terminal-Gly-COSH + Uba4-SH + AMP
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 ?
Urm1p-terminal-Gly-AMP + Uba4-SSH Uba4p first adenylates and then directly transfers sulfur onto Urm1p. Uba4p, consists of an amino N-terminal nucleotide-binding domain (NBD), followed by a MoeZ motif and a carboxy C-terminal rhodanese domain (RHD). Urm1p, adenylated by the nucleotide-binding domain (NBD) of Uba4p, is converted to a Urm1p-thiocarboxylate by Uba4p rhodanese domain (RHD). The sulfur is then passed onto U34 either directly or with the help of Ncs6p/Ncs2p Saccharomyces cerevisiae Urm1p-terminal-Gly-COSH + Uba4-SH + AMP
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Subunits

Subunits Comment Organism
More domain structure of Uba4p, consists of an amino N-terminal nucleotide-binding domain (NBD), followed by a MoeZ motif and a carboxy C-terminal rhodanese domain (RHD). Urm1p, adenylated by the nucleotide-binding domain (NBD) of Uba4p, is converted to a Urm1p-thiocarboxylate by Uba4p rhodanese domain (RHD). The sulfur is then passed onto U34 either directly or with the help of Ncs6p/Ncs2p Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
C-terminal domain of Uba4
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 Saccharomyces cerevisiae
Uba4
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 Saccharomyces cerevisiae