Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-deoxyadenosine | product inhibition. The combination of both products in equimolar concentrations results in more than simple additive inhibition, suggesting that they bind cooperatively to the enzyme. | Escherichia coli | |
L-methionine | product inhibition. The combination of both products in equimolar concentrations results in more than simple additive inhibition, suggesting that they bind cooperatively to the enzyme. | Escherichia coli | |
additional information | 5'-methylthioadenosine is not an inhibitor. Neither biotin nor iminobiotin is a significant inhibitor | Escherichia coli | |
S-adenosyl-L-homocysteine | potent inhibitor | Escherichia coli | |
sinefungin | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
S-adenosyl-L-methionine | in 50 mM Tris-HCl, 100 mM KCl, and 5 mM dithiothreitol, pH 8.0, at 37°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dethiobiotin + [S] + S-adenosyl-L-methionine | - |
Escherichia coli | biotin + L-methionine + 5'-deoxyadenosine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00065 | - |
S-adenosyl-L-homocysteine | in 50 mM Tris-HCl, 100 mM KCl, and 5 mM dithiothreitol, pH 8.0, at 37°C | Escherichia coli | |
0.075 | - |
sinefungin | in 50 mM Tris-HCl, 100 mM KCl, and 5 mM dithiothreitol, pH 8.0, at 37°C | Escherichia coli |