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Literature summary for 2.8.1.6 extracted from

  • Farrar, C.E.; Siu, K.K.; Howell, P.L.; Jarrett, J.T.
    Biotin synthase exhibits burst kinetics and multiple turnovers in the absence of inhibition by products and product-related biomolecules (2010), Biochemistry, 49, 9985-9996.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
5'-deoxyadenosine product inhibition. The combination of both products in equimolar concentrations results in more than simple additive inhibition, suggesting that they bind cooperatively to the enzyme. Escherichia coli
L-methionine product inhibition. The combination of both products in equimolar concentrations results in more than simple additive inhibition, suggesting that they bind cooperatively to the enzyme. Escherichia coli
additional information 5'-methylthioadenosine is not an inhibitor. Neither biotin nor iminobiotin is a significant inhibitor Escherichia coli
S-adenosyl-L-homocysteine potent inhibitor Escherichia coli
sinefungin
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
S-adenosyl-L-methionine in 50 mM Tris-HCl, 100 mM KCl, and 5 mM dithiothreitol, pH 8.0, at 37°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dethiobiotin + [S] + S-adenosyl-L-methionine
-
Escherichia coli biotin + L-methionine + 5'-deoxyadenosine
-
?

Subunits

Subunits Comment Organism
dimer
-
Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00065
-
S-adenosyl-L-homocysteine in 50 mM Tris-HCl, 100 mM KCl, and 5 mM dithiothreitol, pH 8.0, at 37°C Escherichia coli
0.075
-
sinefungin in 50 mM Tris-HCl, 100 mM KCl, and 5 mM dithiothreitol, pH 8.0, at 37°C Escherichia coli