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Literature summary for 2.8.1.6 extracted from

  • Delli-Bovi, T.; Spalding, M.; Prigge, S.
    Overexpression of biotin synthase and biotin ligase is required for efficient generation of sulfur-35 labeled biotin in E. coli (2010), BMC Biotechnol., 10, 73.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli B834(DE3) cells Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dethiobiotin + [S] + 2 S-adenosyl-L-methionine
-
Escherichia coli biotin + 2 L-methionine + 2 5'-deoxyadenosine
-
ir

Synonyms

Synonyms Comment Organism
BioB
-
Escherichia coli

General Information

General Information Comment Organism
physiological function overexpression of biotin synthase is required for efficient generation of sulfur-35 labeled biotin in Escherichia coli. Coexpression of both BioB and the biotin ligase, BirA, is required for efficient biotinylation of biotin carboxy carrier protein-79 Escherichia coli