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Literature summary for 2.8.1.6 extracted from

  • Reyda, M.R.; Fugate, C.J.; Jarrett, J.T.
    A complex between biotin synthase and the iron-sulfur cluster assembly chaperone HscA that enhances in vivo cluster assembly (2009), Biochemistry, 48, 10782-10792.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
hexahistidine tagged protein is expressed in Escherichia coli cells Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
His6-BioB, determined by SDS-PAGE and Western blot analysis Escherichia coli
82630
-
homodimer, theoretical Escherichia coli
90680
-
homodimer, determined by analytical ultracentrifugation Escherichia coli
165300
-
homotetramer, theoretical Escherichia coli
173900
-
homotetramer, determined by analytical ultracentrifugation, non-physiological form Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dethiobiotin + sulfur + S-adenosyl-L-methionine Escherichia coli
-
biotin + L-methionine + 5'-deoxyadenosine
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His6-BioB is purified from Escherichia coli K12 WT strain BW25113, or from knockout strains lacking HscA, HscB, or DnaK, purification on a Ni-NTA-agarose column Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dethiobiotin + sulfur + S-adenosyl-L-methionine
-
Escherichia coli biotin + L-methionine + 5'-deoxyadenosine
-
?

Subunits

Subunits Comment Organism
homodimer
-
Escherichia coli

Synonyms

Synonyms Comment Organism
BioB
-
Escherichia coli
biotin synthase
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
iron-sulfur centre
-
Escherichia coli
S-adenosyl-L-methionine
-
Escherichia coli

General Information

General Information Comment Organism
physiological function biotin synthase catalyses the last step in the biosynthesis of biotin Escherichia coli