Cloned (Comment) | Organism |
---|---|
hexahistidine tagged protein is expressed in Escherichia coli cells | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
His6-BioB, determined by SDS-PAGE and Western blot analysis | Escherichia coli |
82630 | - |
homodimer, theoretical | Escherichia coli |
90680 | - |
homodimer, determined by analytical ultracentrifugation | Escherichia coli |
165300 | - |
homotetramer, theoretical | Escherichia coli |
173900 | - |
homotetramer, determined by analytical ultracentrifugation, non-physiological form | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dethiobiotin + sulfur + S-adenosyl-L-methionine | Escherichia coli | - |
biotin + L-methionine + 5'-deoxyadenosine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His6-BioB is purified from Escherichia coli K12 WT strain BW25113, or from knockout strains lacking HscA, HscB, or DnaK, purification on a Ni-NTA-agarose column | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dethiobiotin + sulfur + S-adenosyl-L-methionine | - |
Escherichia coli | biotin + L-methionine + 5'-deoxyadenosine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
BioB | - |
Escherichia coli |
biotin synthase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
iron-sulfur centre | - |
Escherichia coli | |
S-adenosyl-L-methionine | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | biotin synthase catalyses the last step in the biosynthesis of biotin | Escherichia coli |