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Literature summary for 2.8.1.6 extracted from

  • Lotierzo, M.; Bui, B.T.; Leech, H.K.; Warren, M.J.; Marquet, A.; Rigby, S.E.
    Iron-sulfur cluster dynamics in biotin synthase: a new [2Fe-2S](1+) cluster (2009), Biochem. Biophys. Res. Commun., 381, 487-490.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
hexahistidine tagged proteins are expressed in Escherichia coli BL21DE3 pLysS cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
D155A mutation in the conserved YNHNLD sequence Escherichia coli
H152A mutation in the conserved YNHNLD sequence Escherichia coli
N151A mutation in the conserved YNHNLD sequence Escherichia coli
N153A mutation in the conserved YNHNLD sequence Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dethiobiotin + sulfur + S-adenosyl-L-methionine Escherichia coli
-
biotin + L-methionine + 5'-deoxyadenosine
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dethiobiotin + sulfur + S-adenosyl-L-methionine
-
Escherichia coli biotin + L-methionine + 5'-deoxyadenosine
-
?

Synonyms

Synonyms Comment Organism
BioB
-
Escherichia coli
biotin synthase
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
iron-sulfur centre
-
Escherichia coli
S-adenosyl-L-methionine
-
Escherichia coli

General Information

General Information Comment Organism
physiological function biotin synthase catalyses the final step in the biosynthesis of biotin Escherichia coli