Cloned (Comment) | Organism |
---|---|
The delta pdxH::CAT allele of strain TX2767 is transduced into strain ER47 with phage P1vir to give strain AH14 into which plasmids pER30 and pER35 are introduced | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dethiobiotin + sulfur + 2 S-adenosyl-L-methionine | Escherichia coli | The biotin synthase (BioB) reaction does not require pyridoxal phosphate in vivo. Therefore, the biotin sulfur atom cannot be derived via an intrinsic pyridoxal phosphate-dependent BioB cysteine desulfurase activity | biotin + 2 L-methionine + 2 5'-deoxyadenosine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
host strain is blocked in the synthesis of pyridoxal (due to a null deletion/insertion mutation of pdxH which encodes pyridoxine 5-phosphate oxidase) | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dethiobiotin + sulfur + 2 S-adenosyl-L-methionine | The biotin synthase (BioB) reaction does not require pyridoxal phosphate in vivo. Therefore, the biotin sulfur atom cannot be derived via an intrinsic pyridoxal phosphate-dependent BioB cysteine desulfurase activity | Escherichia coli | biotin + 2 L-methionine + 2 5'-deoxyadenosine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BioB | - |
Escherichia coli |
biotin synthase | - |
Escherichia coli |