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Literature summary for 2.8.1.6 extracted from

  • Taylor, A.M.; Farrar, C.E.; Jarrett, J.T.
    9-Mercaptodethiobiotin is formed as a competent catalytic intermediate by Escherichia coli biotin synthase (2008), Biochemistry, 47, 9309-9317.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Protein Variants

Protein Variants Comment Organism
N153S inactive for biotin production, enzyme can bind AdoMet and DTB and can convert DTB to 9-mercaptodethiobiotin, the majority of 9- mercaptodethiobiotin is released into the buffer and not bound to the enzyme, suggesting that dissociation of 9- mercaptodethiobiotin is faster than the second half reaction for biotin formation Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
dethiobiotin
-
Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dethiobiotin + sulfur + 2 S-adenosyl-L-methionine Escherichia coli 9-mercaptodethiobiotin is formed as a competent catalytic intermediate by Escherichia coli biotin synthase biotin + 2 L-methionine + 2 5'-deoxyadenosine
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin mechanism Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dethiobiotin + sulfur + 2 S-adenosyl-L-methionine 9-mercaptodethiobiotin is formed as a competent catalytic intermediate by Escherichia coli biotin synthase Escherichia coli biotin + 2 L-methionine + 2 5'-deoxyadenosine
-
?

Synonyms

Synonyms Comment Organism
BioB
-
Escherichia coli
biotin synthase
-
Escherichia coli