Protein Variants | Comment | Organism |
---|---|---|
D155A | no enzymic activity, mutant is unable to cleave S-adenosyl-l-methionine and to produce the deoxyadenosyl radical | Escherichia coli |
H152A | about 10% of turnover rate of wild-type. Ratio of 5-deoxyadenosine to biotin is about twice as high as in wild-type | Escherichia coli |
N151A | no enzymic activity, mutant is unable to cleave S-adenosyl-l-methionine and to produce the deoxyadenosyl radical | Escherichia coli |
N153A | no enzymic activity, mutant is unable to cleave S-adenosyl-l-methionine and to produce the deoxyadenosyl radical | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the as-isolated form of enzyme contains an air-stable [2Fe-2S]2+ center. Enzyme can additionally accomodate an air-sensitive [4Fe-4S]2+ center which is generated by incubation under anaerobic conditions with Fe2+ and S2-. With respect to iron cluster content and characteristics, mutants N151A, H152A, N153A, D155A are similar to wild-type | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
recombinant enzyme | - |
Purification (Comment) | Organism |
---|---|
wild-type and mutants N151A, H152A, N153A, D155A. Purified enzymes have a reddish brown colour | Escherichia coli |