Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 1,6-bisphosphate | enhances biotin formation | Arabidopsis thaliana | |
dithiothreitol | required | Arabidopsis thaliana | |
L-cysteine | highly stimulates | Arabidopsis thaliana | |
additional information | one or more unidentified factors from mitochoncrial matrix of pea and potato and from mitochondrial membranes of pea, in addition to the purified enzyme, are obligatory for the conversion of dithiobiotin to biotin in plants | Arabidopsis thaliana | |
S-adenosyl-L-methionine | required | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acidomycin | structural analog of biotin, complete inhibition at 0.4 mM, 50% inhibition at 0.035 mM | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
dethiobiotin | pH 8, 37°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | highly stimulates | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dethiobiotin + sulfur | - |
Arabidopsis thaliana | biotin | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00055 | - |
dethiobiotin | pH 8, 37°C | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | required | Arabidopsis thaliana | |
NADPH | required | Arabidopsis thaliana |