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Literature summary for 2.8.1.6 extracted from

  • Picciocchi, A.; Douce, R.; Alban, C.
    Biochemical characterization of the Arabidopsis biotin synthase reaction. The importance of mitochondria in biotin synthesis (2001), Plant Physiol., 127, 1224-1233.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 1,6-bisphosphate enhances biotin formation Arabidopsis thaliana
dithiothreitol required Arabidopsis thaliana
L-cysteine highly stimulates Arabidopsis thaliana
additional information one or more unidentified factors from mitochoncrial matrix of pea and potato and from mitochondrial membranes of pea, in addition to the purified enzyme, are obligatory for the conversion of dithiobiotin to biotin in plants Arabidopsis thaliana
S-adenosyl-L-methionine required Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
-
Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
acidomycin structural analog of biotin, complete inhibition at 0.4 mM, 50% inhibition at 0.035 mM Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
dethiobiotin pH 8, 37°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ highly stimulates Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dethiobiotin + sulfur
-
Arabidopsis thaliana biotin
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00055
-
dethiobiotin pH 8, 37°C Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADH required Arabidopsis thaliana
NADPH required Arabidopsis thaliana