Cloned (Comment) | Organism |
---|---|
wild-type and mutants | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C188S | enzyme is insoluble | Escherichia coli |
C288T | normal enzyme activity | Escherichia coli |
C53S | no enzyme activity, spectrum shows no peak indicative of the presence of an [Fe-S] cluster | Escherichia coli |
C57S | no enzyme activity, spectrum shows no peak indicative of the presence of an [Fe-S] cluster | Escherichia coli |
C60S | no enzyme activity, spectrum shows no peak indicative of the presence of an [Fe-S] cluster | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38650 | 39670 | wild-type and mutant enzymes, electrospray mass spectrometry | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dethiobiotin + sulfur | - |
Escherichia coli | biotin | - |
? |