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Literature summary for 2.8.1.6 extracted from

  • McIver, L.; Baxter, R.L.; Campopiano, D.J.
    Identification of the [Fe-S] cluster-binding residues of Escherichia coli biotin synthase (2000), J. Biol. Chem., 275, 13888-13894.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutants Escherichia coli

Protein Variants

Protein Variants Comment Organism
C188S enzyme is insoluble Escherichia coli
C288T normal enzyme activity Escherichia coli
C53S no enzyme activity, spectrum shows no peak indicative of the presence of an [Fe-S] cluster Escherichia coli
C57S no enzyme activity, spectrum shows no peak indicative of the presence of an [Fe-S] cluster Escherichia coli
C60S no enzyme activity, spectrum shows no peak indicative of the presence of an [Fe-S] cluster Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38650 39670 wild-type and mutant enzymes, electrospray mass spectrometry Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dethiobiotin + sulfur
-
Escherichia coli biotin
-
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