Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
FeCl3 | - |
Escherichia coli | |
FeCl3 | enhances activity 3-4fold at 0.1 mM | Lysinibacillus sphaericus | |
Iron | the enzyme contains an iron-sulfur cluster | Escherichia coli | |
Iron | the enzyme contains an iron-sulfur cluster | Lysinibacillus sphaericus | |
Na2S | - |
Escherichia coli | |
Na2S | enhances activity 3-4fold at 1 mM | Lysinibacillus sphaericus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dethiobiotin + sulfur | Escherichia coli | hypothesis: sulfur of biotin is derived from the [Fe-S] center of the enzyme | biotin | - |
? | |
dethiobiotin + sulfur | Lysinibacillus sphaericus | hypothesis: sulfur of biotin is derived from the [Fe-S] center of the enzyme | biotin | - |
? | |
dethiobiotin + sulfur | Escherichia coli | catalyzes the last step of the biosynthesis of biotin | biotin | - |
? | |
dethiobiotin + sulfur | Lysinibacillus sphaericus | catalyzes the last step of the biosynthesis of biotin | biotin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Lysinibacillus sphaericus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin | mechanism | Escherichia coli | |
dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin | mechanism | Lysinibacillus sphaericus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dethiobiotin + sulfur | - |
Escherichia coli | biotin | - |
? | |
dethiobiotin + sulfur | - |
Lysinibacillus sphaericus | biotin | - |
? | |
dethiobiotin + sulfur | hypothesis: sulfur of biotin is derived from the [Fe-S] center of the enzyme | Escherichia coli | biotin | - |
? | |
dethiobiotin + sulfur | hypothesis: sulfur of biotin is derived from the [Fe-S] center of the enzyme | Lysinibacillus sphaericus | biotin | - |
? | |
dethiobiotin + sulfur | catalyzes the last step of the biosynthesis of biotin | Escherichia coli | biotin | - |
? | |
dethiobiotin + sulfur | catalyzes the last step of the biosynthesis of biotin | Lysinibacillus sphaericus | biotin | - |
? |