KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
dethiobiotin | pH 7.5 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the 104000 Da dimeric enzyme form contains a single [2Fe-2S] cluster per dimer | Escherichia coli | |
Iron | the 82000 Da dimeric enzyme form contains one [2Fe-2S]cluster per monomer | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38700 | - |
2 * 38700, calculation from nucleotide sequence | Escherichia coli |
39000 | - |
2 * 39000, 82000 MW form and 104000 MW form, SDS-PAGE | Escherichia coli |
82000 | - |
gel electrophoresis | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dethiobiotin + sulfur | - |
Escherichia coli | biotin | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38700, calculation from nucleotide sequence | Escherichia coli |
dimer | 2 * 39000, 82000 MW form and 104000 MW form, SDS-PAGE | Escherichia coli |