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Literature summary for 2.8.1.6 extracted from
- Biotin synthase of Bacillus sphaericus (1997), Methods Enzymol., 279, 356-362.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | - |
Lysinibacillus sphaericus |  |
| L-cysteine | - |
Lysinibacillus sphaericus | |
| S-adenosyl-L-methionine | absolute requirement as electron source | Lysinibacillus sphaericus | |
General Stability
| General Stability | Organism |
|---|---|
| loses all its clusters if the purification is carried out with buffers that have not been saturated with argon | Lysinibacillus sphaericus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | above 1 mM | Lysinibacillus sphaericus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0016 | - |
dethiobiotin | - |
Lysinibacillus sphaericus | |
| 0.005 | - |
9-Mercaptodethiobiotin | - |
Lysinibacillus sphaericus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | presence of a [2Fe-2S] cluster | Lysinibacillus sphaericus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36954 | - |
2 * 36954, calculation from nucleotide sequence | Lysinibacillus sphaericus |
| 37000 | - |
2 * 37000, SDS-PAGE | Lysinibacillus sphaericus |
| 75000 | - |
gel filtration | Lysinibacillus sphaericus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lysinibacillus sphaericus | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| oxygen sensitive | Lysinibacillus sphaericus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lysinibacillus sphaericus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Lysinibacillus sphaericus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 9-mercaptodethiobiotin + sulfur | - |
Lysinibacillus sphaericus | biotin | - |
? | |
| dethiobiotin + sulfur | ultimate sulfur donor has not yet been identified | Lysinibacillus sphaericus | biotin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 37000, SDS-PAGE | Lysinibacillus sphaericus |
| dimer | 2 * 36954, calculation from nucleotide sequence | Lysinibacillus sphaericus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Lysinibacillus sphaericus | |
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Release 2023.1 (January 2023)
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