Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mus musculus |
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D63A | mitochondrial splice variant MPST2, mutation of catalytic triad, mutation of catalytic triad, specific activity in presence of cysteine is comparable to wild-type, specific activity with thioredoxin is 2.3fold decreased | Mus musculus |
D63A | mitochondrial splice variant MPST2, mutation of catalytic triad, mutation of catalytic triad, specific activity in presence of cysteine is comparable to wild-type, specific activity with thioredoxin is 2.3fold decreased | Homo sapiens |
H74A | mitochondrial splice variant MPST2, mutation of catalytic triad, specific activity in presence of cysteine is comparable to wild-type, specific activity with thioredoxin is 1.9fold decreased | Mus musculus |
H74A | mitochondrial splice variant MPST2, mutation of catalytic triad, specific activity in presence of cysteine is comparable to wild-type, specific activity with thioredoxin is 1.9fold decreased | Homo sapiens |
S250A | mitochondrial splice variant MPST2, mutation of catalytic triad, specific activity with cysteine is 2.6fold lower than wild-type, with thioredoxin, specific activity is similar to wild-type | Mus musculus |
S250A | mitochondrial splice variant MPST2, mutation of catalytic triad, specific activity with cysteine is 2.6fold lower than wild-type, with thioredoxin, specific activity is similar to wild-type | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | substrate inhibition of mitochondrial splice variant MPST2 | Homo sapiens | |
thioredoxin | substrate inhibition of mitochondrial splice variant MPST2 | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
thioredoxin | cytosolic splice variant MPST1, Hill coefficient 1.6, pH 7.4, 37°C | Homo sapiens | |
0.0031 | - |
thioredoxin | mitochondrial splice variant MPST2, Hill coefficient 1.4, pH 7.4, 37°C | Homo sapiens | |
0.015 | - |
3-Mercaptopyruvate | cosubstrate N-acetyl-L-cysteine, mitochondrial splice variant MPST2, Hill coefficient 1.1, pH 7.4, 37°C | Homo sapiens | |
0.02 | - |
3-Mercaptopyruvate | cosubstrate N-acetyl-L-cysteine, cytosolic splice variant MPST1, Hill coefficient 1.3, pH 7.4, 37°C | Homo sapiens | |
0.022 | - |
3-Mercaptopyruvate | cosubstrate L-cysteine, mitochondrial splice variant MPST2, Hill coefficient 1.4, pH 7.4, 37°C | Homo sapiens | |
0.024 | - |
3-Mercaptopyruvate | cosubstrate L-cysteine, cytosolic splice variant MPST1, Hill coefficient 1.6, pH 7.4, 37°C | Homo sapiens | |
0.358 | - |
3-Mercaptopyruvate | cosubstrate thioredoxin, cytosolic splice variant MPST1, Hill coefficient 2.0, pH 7.4, 37°C | Homo sapiens | |
0.36 | - |
3-Mercaptopyruvate | cosubstrate thioredoxin, mitochondrial splice variant MPST2, Hill coefficient 2.1, pH 7.4, 37°C | Homo sapiens | |
3.8 | - |
L-cysteine | cytosolic splice variant MPST1, Hill coefficient 1.4, pH 7.4, 37°C | Homo sapiens | |
4.5 | - |
L-cysteine | mitochondrial splice variant MPST2, Hill coefficient 1.6, pH 7.4, 37°C | Homo sapiens | |
16 | - |
N-acetyl-L-cysteine | mitochondrial splice variant MPST2, Hill coefficient 2.7, pH 7.4, 37°C | Homo sapiens | |
17 | - |
N-acetyl-L-cysteine | cytosolic splice variant MPST1, Hill coefficient 2.2, pH 7.4, 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | two splice variants of MPST, differing by 20 amino acids at the N-terminus, give rise to the cytosolic MPST1 and mitochondrial MPST2 isoforms | Homo sapiens | 5829 | - |
mitochondrion | two splice variants of MPST, differing by 20 amino acids at the N-terminus, give rise to the cytosolic MPST1 and mitochondrial MPST2 isoforms | Homo sapiens | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P25325 | - |
- |
Mus musculus | Q99J99 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | 10fold lower activity than in liver | Mus musculus | - |
kidney | 2fold lower activity than in liver | Mus musculus | - |
liver | highest activity observed | Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.7 | - |
mitochondrial splice variant MPST2, mutant S250A, substrate L-cysteine, pH 7.4, 37°C | Homo sapiens |
1 | - |
mitochondrial splice variant MPST2, mutant D63A, substrate thioredoxin, pH 7.4, 37°C | Homo sapiens |
1.2 | - |
mitochondrial splice variant MPST2, mutant H74A, substrate thioredoxin, pH 7.4, 37°C | Homo sapiens |
1.4 | - |
mitochondrial splice variant MPST2, mutant D63A, substrate L-cysteine, pH 7.4, 37°C | Homo sapiens |
1.6 | - |
mitochondrial splice variant MPST2, mutant H74A, substrate L-cysteine, pH 7.4, 37°C | Homo sapiens |
1.8 | - |
wild-type mitochondrial splice variant MPST2, substrate L-cysteine, pH 7.4, 37°C | Homo sapiens |
2.3 | - |
mitochondrial splice variant MPST2, mutant S250A, substrate thioredoxin, pH 7.4, 37°C | Homo sapiens |
2.3 | - |
wild-type mitochondrial splice variant MPST2, substrate thioredoxin, pH 7.4, 37°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-mercaptopyruvate + L-cysteine | - |
Mus musculus | ? | - |
? | |
3-mercaptopyruvate + L-cysteine | - |
Homo sapiens | ? | - |
? | |
3-mercaptopyruvate + N-acetyl-L-cysteine | - |
Mus musculus | ? | - |
? | |
3-mercaptopyruvate + N-acetyl-L-cysteine | - |
Homo sapiens | ? | - |
? | |
3-mercaptopyruvate + thioredoxin | - |
Mus musculus | pyruvate + persulfurated thioredoxin | - |
? | |
3-mercaptopyruvate + thioredoxin | - |
Homo sapiens | pyruvate + persulfurated thioredoxin | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
N-acetyl-L-cysteine | cytosolic splice variant MPST1, Hill coefficient 2.2, pH 7.4, 37°C | Homo sapiens | |
0.27 | - |
N-acetyl-L-cysteine | mitochondrial splice variant MPST2, Hill coefficient 2.2, pH 7.4, 37°C | Homo sapiens | |
0.4 | - |
thioredoxin | cytosolic splice variant MPST1, Hill coefficient 1.6, pH 7.4, 37°C | Homo sapiens | |
0.44 | - |
L-cysteine | cytosolic splice variant MPST1, Hill coefficient 1.4, pH 7.4, 37°C | Homo sapiens | |
0.44 | - |
thioredoxin | mitochondrial splice variant MPST2, Hill coefficient 1.6, pH 7.4, 37°C | Homo sapiens | |
0.5 | - |
L-cysteine | mitochondrial splice variant MPST2, Hill coefficient 1.4, pH 7.4, 37°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
N-acetyl-L-cysteine | cytosolic splice variant MPST1, Hill coefficient 2., pH 7.4, 37°C | Homo sapiens | |
0.017 | - |
N-acetyl-L-cysteine | mitochondrial splice variant MPST2, Hill coefficient 2.2, pH 7.4, 37°C | Homo sapiens | |
0.11 | - |
L-cysteine | cytosolic splice variant MPST1, Hill coefficient 1.4, pH 7.4, 37°C | Homo sapiens | |
0.11 | - |
L-cysteine | mitochondrial splice variant MPST2, Hill coefficient 1.4, pH 7.4, 37°C | Homo sapiens | |
0.44 | - |
thioredoxin | mitochondrial splice variant MPST2, Hill coefficient 1.6, pH 7.4, 37°C | Homo sapiens | |
133 | - |
thioredoxin | cytosolic splice variant MPST1, Hill coefficient 1.6, pH 7.4, 37°C | Homo sapiens |