Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 2 M ammonium sulfate, 0.1 M sodium acetate pH 4.5 and 0.02 M betaine hydrochloride | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
thioredoxin | in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
0.02 | - |
3-Mercaptopyruvate | glutathione as cosubstrate, in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
0.022 | - |
3-Mercaptopyruvate | using L-cysteine as cosubstrate, in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
0.025 | - |
3-Mercaptopyruvate | using dihydrolipioic acid as cosubstrate, in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
0.026 | - |
3-Mercaptopyruvate | using dithiothreitol as cosubstrate, in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
0.03 | - |
3-Mercaptopyruvate | using L-homocysteine as cosubstrate, in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
0.13 | - |
3-Mercaptopyruvate | using 2-mercaptoethanol as cosubstrate, in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
0.35 | - |
3-Mercaptopyruvate | using cyanide as cosubstrate, in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
0.35 | - |
3-Mercaptopyruvate | using thioredoxin as cosubstrate, in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
4.1 | - |
L-cysteine | in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
4.4 | - |
dihydrolipoic acid | in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
4.6 | - |
dithiothreitol | in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
6 | - |
cyanide | in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
12.5 | - |
L-homocysteine | in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
28 | - |
glutathione | in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
108 | - |
2-mercaptoethanol | in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-mercaptopyruvate + cyanide | Homo sapiens | - |
pyruvate + thiocyanate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P25325 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography and DEAE-Sepharose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-mercaptopyruvate + 2-mercaptoethanol | - |
Homo sapiens | ? | - |
? | |
3-mercaptopyruvate + cyanide | - |
Homo sapiens | pyruvate + thiocyanate | - |
? | |
3-mercaptopyruvate + dihydrolipoic acid | - |
Homo sapiens | ? | - |
? | |
3-mercaptopyruvate + dithiothreitol | - |
Homo sapiens | ? | - |
? | |
3-mercaptopyruvate + glutathione | - |
Homo sapiens | ? | - |
? | |
3-mercaptopyruvate + L-cysteine | - |
Homo sapiens | ? | - |
? | |
3-mercaptopyruvate + L-homocysteine | - |
Homo sapiens | ? | - |
? | |
3-mercaptopyruvate + thioredoxin | thioredoxin is the preferred persulfide acceptor | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
mercaptopyruvate sulfurtransferase | - |
Homo sapiens |
MST | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
glutathione | in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
0.8 | - |
L-homocysteine | in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
1.1 | - |
L-cysteine | in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
1.3 | - |
thioredoxin | in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
1.7 | - |
dihydrolipoic acid | in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
2.4 | - |
cyanide | in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
6.1 | - |
dithiothreitol | in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
229 | - |
2-mercaptoethanol | in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
glutathione | in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
0.06 | - |
L-homocysteine | in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
0.27 | - |
L-cysteine | in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
0.39 | - |
dihydrolipoic acid | in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
0.4 | - |
cyanide | in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
1.3 | - |
dithiothreitol | in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
2.1 | - |
2-mercaptoethanol | in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens | |
520 | - |
thioredoxin | in 200 mM of HEPES, pH 7.4, at 37°C | Homo sapiens |