Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | activates MST chiefly via reduction of a sulfenyl Cys247 | Rattus norvegicus | |
additional information | reduced glutathione does not affect MST activity | Rattus norvegicus | |
thioredoxin | a low redox potential sulfenate is reversibly formed at a catalytic site cysteine so as to inhibit MST, and thioredoxin-dependent reduction of the sulfenate restored the MST activity | Rattus norvegicus | |
thioredoxin | an intermolecular disulfide bond serves as a thioredoxin-dependent redox-sensing switch for the regulation of the enzymatic activity of 3-mercaptopyruvate sulfurtransferase. A cysteine residue on the surface of each subunit is oxidized to form an intersubunit disulfide bond so as to decrease MST activity, and thioredoxin-specific conversion of a dimer to a monomer increased MST acitvity | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32800 | - |
monomer, HPLC analysis, MST exhibits monomer-dimer equilibrium, monomer to dimer ratio is nearly 9 to 1 in 0.2 M potassium phosphate buffer, pH 7.0 | Rattus norvegicus |
66600 | - |
dimer, HPLC analysis, MST exhibits monomer-dimer equilibrium, the dimer is formed via intersubunit disulfide bond, inactive form | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | MST contributes to maintain redox homeostasis via exerting control over cysteine catabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P97532 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | MST contributes to maintain redox homeostasis via exerting control over cysteine catabolism | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2*32800, HPLC, MST exhibits monomer-dimer equilibrium, the dimer is formed via intersubunit disulfide bond, inactive form | Rattus norvegicus |
monomer | 32800, HPLC, MST exhibits monomer-dimer equilibrium | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
3-mercaptopyruvate sulfurtransferase | - |
Rattus norvegicus |
mercaptopyruvate sulfurtransferase | - |
Rattus norvegicus |
MST | - |
Rattus norvegicus |