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Literature summary for 2.8.1.2 extracted from

  • Nagahara, N.
    A novel mercaptopyruvate sulfurtransferase thioredoxin-dependent redox-sensing molecular switch: a mechanism for the maintenance of cellular redox equilibrium (2008), Mini Rev. Med. Chem., 8, 585-589.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol activates MST chiefly via reduction of a sulfenyl Cys247 Rattus norvegicus
additional information reduced glutathione does not affect MST activity Rattus norvegicus
thioredoxin a low redox potential sulfenate is reversibly formed at a catalytic site cysteine so as to inhibit MST, and thioredoxin-dependent reduction of the sulfenate restored the MST activity Rattus norvegicus
thioredoxin an intermolecular disulfide bond serves as a thioredoxin-dependent redox-sensing switch for the regulation of the enzymatic activity of 3-mercaptopyruvate sulfurtransferase. A cysteine residue on the surface of each subunit is oxidized to form an intersubunit disulfide bond so as to decrease MST activity, and thioredoxin-specific conversion of a dimer to a monomer increased MST acitvity Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32800
-
monomer, HPLC analysis, MST exhibits monomer-dimer equilibrium, monomer to dimer ratio is nearly 9 to 1 in 0.2 M potassium phosphate buffer, pH 7.0 Rattus norvegicus
66600
-
dimer, HPLC analysis, MST exhibits monomer-dimer equilibrium, the dimer is formed via intersubunit disulfide bond, inactive form Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus MST contributes to maintain redox homeostasis via exerting control over cysteine catabolism ?
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P97532
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information MST contributes to maintain redox homeostasis via exerting control over cysteine catabolism Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
dimer 2*32800, HPLC, MST exhibits monomer-dimer equilibrium, the dimer is formed via intersubunit disulfide bond, inactive form Rattus norvegicus
monomer 32800, HPLC, MST exhibits monomer-dimer equilibrium Rattus norvegicus

Synonyms

Synonyms Comment Organism
3-mercaptopyruvate sulfurtransferase
-
Rattus norvegicus
mercaptopyruvate sulfurtransferase
-
Rattus norvegicus
MST
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Rattus norvegicus