KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
73 | - |
3-Mercaptopyruvate | pH 9.6 | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
1 * 34000, enzyme exists as a monomer and homodimer, SDS-PAGE, gel filtration | Rattus norvegicus |
34000 | - |
2 * 34000, enzyme exists as a monomer and homodimer, SDS-PAGE, gel filtration | Rattus norvegicus |
34500 | - |
two peaks with molecular masses of 34500 Da and 53500 Da, enzyme exists as a monomer and homodimer, gel filtration | Rattus norvegicus |
53000 | - |
two peaks with molecular masses of 34500 Da and 53500 Da, enzyme exists as a monomer and homodimer, gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-mercaptopyruvate + cyanide | Rattus norvegicus | - |
pyruvate + thiocyanate | - |
? | |
3-mercaptopyruvate + cyanide | Rattus norvegicus Wistar | - |
pyruvate + thiocyanate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Wistar | - |
Rattus norvegicus Wistar | - |
Wistar | - |
Purification (Comment) | Organism |
---|---|
homogeneity | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | predominantly in pericentral hepatocytes | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3793 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-mercaptopyruvate + cyanide | - |
Rattus norvegicus | pyruvate + thiocyanate | - |
? | |
3-mercaptopyruvate + cyanide | - |
Rattus norvegicus Wistar | pyruvate + thiocyanate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 34000, enzyme exists as a monomer and homodimer, SDS-PAGE, gel filtration | Rattus norvegicus |
monomer | 1 * 34000, enzyme exists as a monomer and homodimer, SDS-PAGE, gel filtration | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
25 | - |
3-Mercaptopyruvate | pH 9.55 | Rattus norvegicus |