Any feedback?
Literature summary for 2.8.1.2 extracted from
- Mercaptopyruvate sulfurtransferase as a defense against cyanide toxication: molecular properties and mode of detoxification (1999), Histol. Histopathol., 14, 1277-1286.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| liver enzyme | Rattus norvegicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R196G | decrease in kcat/Km | Rattus norvegicus |
| S249K | decrease in kcat/Km | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Rattus norvegicus | 5737 | - |
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-mercaptopyruvate + cyanide | Rattus norvegicus | - |
pyruvate + thiocyanate | - |
? |  |
| additional information | Rattus norvegicus | cyanide detoxification | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| homogeneity | Rattus norvegicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 3-mercaptopyruvate + reduced thioredoxin = pyruvate + hydrogen sulfide + oxidized thioredoxin | mechanism | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| cerebellum | - |
Rattus norvegicus | - |
| cerebrum | - |
Rattus norvegicus | - |
| glial cell | - |
Rattus norvegicus | - |
| heart | - |
Rattus norvegicus | - |
| kidney | predominantly in tubular epithelium | Rattus norvegicus | - |
| liver | predominantly in pericentral hepatocytes | Rattus norvegicus | - |
| lung | - |
Rattus norvegicus | - |
| testis | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-mercaptopyruvate + cyanide | - |
Rattus norvegicus | pyruvate + thiocyanate | - |
? | |
| additional information | cyanide detoxification | Rattus norvegicus | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0783 | - |
3-Mercaptopyruvate | S249K mutant | Rattus norvegicus | |
| 0.233 | - |
3-Mercaptopyruvate | G248R mutant | Rattus norvegicus | |
| 0.917 | - |
3-Mercaptopyruvate | S249A mutant | Rattus norvegicus | |
| 1.03 | - |
3-Mercaptopyruvate | wild-type enzyme | Rattus norvegicus | |
| 1.07 | - |
3-Mercaptopyruvate | R196G mutant | Rattus norvegicus | |
| 5.67 | - |
3-Mercaptopyruvate | R187G mutant | Rattus norvegicus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
