Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor | Thermus thermophilus | - |
AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor | - |
? | |
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor | Thermus thermophilus DSM 7039 | - |
AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q72LF3 | - |
- |
Thermus thermophilus DSM 7039 | Q72LF3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor | - |
Thermus thermophilus | AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor | - |
? | |
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor | - |
Thermus thermophilus DSM 7039 | AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TtuA | - |
Thermus thermophilus |
TT_C0106 | - |
Thermus thermophilus |
Veg136 protein | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Thermus thermophilus |
General Information | Comment | Organism |
---|---|---|
metabolism | The enzyme is required for growth at temperatures above 75°C. Thiolation of thymidine54 in tRNA results in a tRNA molecule with higher thermostability | Thermus thermophilus |