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Literature summary for 2.8.1.13 extracted from

  • Noma, A.; Sakaguchi, Y.; Suzuki, T.
    Mechanistic characterization of the sulfur-relay system for eukaryotic 2-thiouridine biogenesis at tRNA wobble positions (2009), Nucleic Acids Res., 37, 1335-1352.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.1.13

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
TusE-SSH + adenylated-tRNA-uridine Escherichia coli the biogenesis of 2-thiouridine in Escherichia coli utilizes persulfide chemistry and proceeds through a complex sulfur-relay system by multiple sulfur mediators that select and facilitate specific sulfur flow to 2-thiouridine from various cellular sulfur pathways. TusE interacts with MnmA, a thiouridylase responsible for 2-thiouridine formation, and transfers the persulfide to it. MnmA activates the C2-position of the uracil base at the wobble position of the tRNA by forming an acyl-adenylated intermediate. Then, nucleophilic attack by the persulfide sulfur on the activated carbon generates a thiocarbonyl group by releasing the AMP TusE-SH + tRNA-2-thiouridine + AMP
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Organism

Organism UniProt Comment Textmining
Escherichia coli P25745
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
TusE-SSH + adenylated-tRNA-uridine the biogenesis of 2-thiouridine in Escherichia coli utilizes persulfide chemistry and proceeds through a complex sulfur-relay system by multiple sulfur mediators that select and facilitate specific sulfur flow to 2-thiouridine from various cellular sulfur pathways. TusE interacts with MnmA, a thiouridylase responsible for 2-thiouridine formation, and transfers the persulfide to it. MnmA activates the C2-position of the uracil base at the wobble position of the tRNA by forming an acyl-adenylated intermediate. Then, nucleophilic attack by the persulfide sulfur on the activated carbon generates a thiocarbonyl group by releasing the AMP Escherichia coli TusE-SH + tRNA-2-thiouridine + AMP
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 ?
TusE-SSH + adenylated-tRNA-uridine TusE interacts with MnmA, a thiouridylase responsible for 2-thiouridine formation, and transfers the persulfide to it. MnmA activates the C2-position of the uracil base at the wobble position of the tRNA by forming an acyl-adenylated intermediate. Then, nucleophilic attack by the persulfide sulfur on the activated carbon generates a thiocarbonyl group by releasing the AMP Escherichia coli TusE-SH + tRNA-2-thiouridine + AMP
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 ?

Synonyms

Synonyms Comment Organism
MnmA
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 Escherichia coli