Crystallization (Comment) | Organism |
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hanging-drop vapour-diffusion method, the binary complex of MnmA and tRNAGlu is crystallized in two different crystal forms: forms I and II. Cocrystallization of MnmAtRNAGlu with ATP yields form III crystals. The three crystal forms diffracted to 3.1 A, 3.4 A and 3.4 A resolution, respectively | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
TusE-SSH + adenylated-tRNA-uridine | Escherichia coli | MnmA catalyzes a sulfuration reaction to synthesize 2-thiouridine at the wobble positions of tRNAGlu, tRNAGln and tRNALys in Escherichia coli | TusE-SH + tRNA-2-thiouridine + AMP | - |
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Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P25745 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
TusE-SSH + adenylated-tRNA-uridine | - |
Escherichia coli | TusE-SH + tRNA-2-thiouridine + AMP | - |
? | |
TusE-SSH + adenylated-tRNA-uridine | MnmA catalyzes a sulfuration reaction to synthesize 2-thiouridine at the wobble positions of tRNAGlu, tRNAGln and tRNALys in Escherichia coli | Escherichia coli | TusE-SH + tRNA-2-thiouridine + AMP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MnmA | - |
Escherichia coli |