Literature summary for 2.8.1.13 extracted from

Numata, T.; Ikeuchi, Y.; Fukai, S.; Suzuki, T.; Nureki, O.
Snapshots of tRNA sulphuration via an adenylated intermediate (2006), Nature, 442, 419-424.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structures of the MnmA thiouridylasetRNA(Glu) binary complex in three discrete forms, which provide snapshots of the sequential chemical reactions during RNA sulfuration	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P25745	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
a [protein]-S-sulfanyl-L-cysteine + uacil34 in tRNA + ATP + reduced acceptor = a [protein]-L-cysteine + 2-thiouracil34 in tRNA + AMP + diphosphate + acceptor	on enzyme activation, an alpha-helix overhanging the active site is restructured into an idiosyncratic beta-hairpin-containing loop, which packs the flipped-out U34 deeply into the catalytic pocket and triggers the activation of the catalytic cysteine residues. The adenylated RNA intermediate is trapped. The active closed-conformation of the complex ensures accurate sulfur incorporation into the activated uridine carbon by forming a catalytic chamber to prevent solvent from accessing the catalytic site	Escherichia coli	a

Synonyms

Synonyms	Comment	Organism
MnmA	-	Escherichia coli

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Release 2023.1 (January 2023)