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Literature summary for 2.8.1.13 extracted from
- MnmA and IscS are required for in vitro 2-thiouridine biosynthesis in Escherichia coli (2003), Biochemistry, 42, 1109-1117.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
General Stability
| General Stability | Organism |
|---|---|
| ATP protects MnmA from trypsinolysis | Escherichia coli |
| tRNA(Glu) protects MnmA from tryptic digestion | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli | thionucleosides are uniquely present in tRNA. In many organisms, tRNAs specific for Lys, Glu, and Gln contain hypermodified 2-thiouridine (s2U) derivatives at wobble position 34. The s2 group of s2U34 stabilizes anticodon structure, confers ribosome binding ability to tRNA and improves reading frame maintenance | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P25745 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | thionucleosides are uniquely present in tRNA. In many organisms, tRNAs specific for Lys, Glu, and Gln contain hypermodified 2-thiouridine (s2U) derivatives at wobble position 34. The s2 group of s2U34 stabilizes anticodon structure, confers ribosome binding ability to tRNA and improves reading frame maintenance | Escherichia coli | ? | - |
? | |
| additional information | thionucleosides are uniquely present in tRNA. In many organisms, tRNAs specific for Lys, Glu, and Gln contain hypermodified 2-thiouridine (s2U) derivatives at wobble position 34. Purified IscS-persulfide is able to provide sulfur for in vitro s2U synthesis in the absence of cysteine. The s2 group of s2U34 stabilizes anticodon structure, confers ribosome binding ability to tRNA and improves reading frame maintenance. Identification of 2-thiouridine as product of the in vitro s2U reaction. | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MnmA | - |
Escherichia coli |
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