Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Oryza sativa Japonica Group | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Oryza sativa Japonica Group |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + phosphate | Oryza sativa Japonica Group | - |
AMP + phosphoenolpyruvate + diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa Japonica Group | Q75KR1 | cultivars 9311 and TXZ-25 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | rapid phosphorylation mechanism of the enzyme, phosphorylation at residue Thr527 of PPDK. The phosphorylation rate of the enzyme is increased at higher temperature of 31°C compared to control at 24°C | Oryza sativa Japonica Group |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + phosphate | - |
Oryza sativa Japonica Group | AMP + phosphoenolpyruvate + diphosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
Cyppdk | - |
Oryza sativa Japonica Group |
cytosolic pyruvate orthophosphate dikinase | - |
Oryza sativa Japonica Group |
PPDK2 | - |
Oryza sativa Japonica Group |
pyruvate orthophosphate dikinase | - |
Oryza sativa Japonica Group |
pyruvate, phosphate dikinase 2 | - |
Oryza sativa Japonica Group |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Oryza sativa Japonica Group |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Oryza sativa Japonica Group |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Oryza sativa Japonica Group | |
ATP | - |
Oryza sativa Japonica Group |
Organism | Comment | Expression |
---|---|---|
Oryza sativa Japonica Group | a reduction in PPDK activities due to high temperature (31°C) is observed during the middle and late grain filling periods, accompanied by downregulated cyPPDK mRNA and protein levels, temperature effects on the developmental regulation of PPDK activity are confirmed at transcription, translation and post-translational levels | down |
General Information | Comment | Organism |
---|---|---|
malfunction | a reduction in PPDK activities due to high temperature (31°C) is observed during the middle and late grain filling periods, accompanied by downregulated cyPPDK mRNA and protein levels, leading to high temperature-induced chalkiness. Rice chalkiness due to high temperature during grain filling lower the grain quality. Temperature effects on the developmental regulation of PPDK activity are confirmed at transcription, translation and post-translational levels. Comparison of rice grain types at 24°C and 31°C of the two cultivars, overview | Oryza sativa Japonica Group |
additional information | PPDK activities are insensitive to variation in PPDK levels, suggesting the rapid phosphorylation mechanism of this protein. The phosphorylation rate of the enzyme is increased at higher temperature of 31°C compared to control at 24°C | Oryza sativa Japonica Group |
physiological function | a function of isozyme cyPPDK in rice is to modulate carbon metabolism during grain filling | Oryza sativa Japonica Group |