Literature summary for 2.7.9.1 extracted from

Saidin, S.; Yunus, M.H.; Zakaria, N.D.; Razak, K.A.; Huat, L.B.; Othman, N.; Noordin, R.
Production of recombinant Entamoeba histolytica pyruvate phosphate dikinase and its application in a lateral flow dipstick test for amoebic liver abscess (2014), BMC Infect. Dis., 14, 182.

Search for more literature for 2.7.9.1

Application

Application	Comment	Organism
diagnostics	the enzyme is a potential diagnostic marker for amoebic liver abscess. Development of a lateral flow dipstick (LFD) test using recombinant PPDK enzyme and evaluation for serodiagnosis of amoebic liver abscess, enzyme detection using mouse anti-human IgG4, directed against the parasite enzyme, conjugated to colloidal gold, overview	Entamoeba histolytica

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of soluble His-tagged enzyme in Escherichia coli strain BL21(DE3)	Entamoeba histolytica

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	pyruvate phosphate dikinase is an excretory-secretory protein	Entamoeba histolytica	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
98000	-	-	Entamoeba histolytica

Organism

Organism	UniProt	Comment	Textmining
Entamoeba histolytica	P37213	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged soluble enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration	Entamoeba histolytica

Source Tissue

Source Tissue	Comment	Organism	Textmining

Subunits

Subunits	Comment	Organism
?	x * 98000, recombinant His-tagged enzyme, SDS-PAGE	Entamoeba histolytica

Synonyms

Synonyms	Comment	Organism
PPDK	-	Entamoeba histolytica
pyruvate phosphate dikinase	-	Entamoeba histolytica

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Release 2023.1 (January 2023)