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Literature summary for 2.7.9.1 extracted from

  • Arakawa, H.; Karasawa, K.; Munakata, E.; Obinata, R.; Maeda, M.; Suzuki, S.; Kamahori, M.; Kambara, H.
    Development of bioluminescent pyrophosphate assay using pyruvate phosphate dikinase and its application to single-nucleotide polymorphism analysis (2008), Anal. Biochem., 379, 86-90.
    View publication on PubMed
Search for more literature for 2.7.9.1

Application

Application Comment Organism
analysis PPDK is useful in DNA analysis, method development, overview Microbispora rosea

Protein Variants

Protein Variants Comment Organism
additional information development of a bioluminescent assay method for diphosphate, detection applied to single-nucleotide polymorphism analysis using one-base extension reaction, overview Microbispora rosea

Organism

Organism UniProt Comment Textmining
Microbispora rosea
-
 subsp. aer­ata
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Microbispora rosea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AMP + phosphoenolpyruvate + diphosphate diphosphate released by DNA polymerase is converted to ATP by pyruvate phosphate dikinase, PPDK Microbispora rosea ATP + pyruvate + phosphate
-
 r

Synonyms

Synonyms Comment Organism
PPDK
-
 Microbispora rosea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Microbispora rosea

Cofactor

Cofactor Comment Organism Structure
AMP forward reaction Microbispora rosea
ATP reverse reaction Microbispora rosea