Application | Comment | Organism |
---|---|---|
agriculture | modification of the cold sensitivity of a C4 photosynthetic enzyme by employing cereal transformation technology. A significant improvement in the cold stability of PPDK can be achieved when a suffcient quantity of cold-tolerant subunits is expressed in transgenic maize leaves | Zea mays |
Cloned (Comment) | Organism |
---|---|
high levels of expression in Zea mays by using a double intron cassette and a chimeric cDNA made from Flaveria bidentis and Flaveria brownii with a maximum content of 1 mg/g fresh weight. In leaves of transgenic maize, PPDK molecules produced from the transgene are detected in cold-tolerant homotetramers or in heterotetramers of intermediate cold susceptibility formed with the internal PPDK | Flaveria bidentis |
introduction of a cold-tolerant PPDK cDNA isolated from Flaveria brownii into maize by Agrobacterium-mediated transformation. Higher levels of expression by using a double intron cassette and a chimeric cDNA made from Flaveria bidentis and Flaveria brownii with a maximum content of 1 mg/g fresh weight. In leaves of transgenic maize, PPDK molecules produced from the transgene are detected in cold-tolerant homotetramers or in heterotetramers of intermediate cold susceptibility formed with the internal PPDK | Flaveria brownii |
to improve the cold stability of the enzyme, a cold-tolerant PPDK cDNA isolated from Flaveria brownii is introduced into maize by Agrobacterium-mediated transformation. Higher levels of expression are ontained by using a double intron cassette and a chimeric cDNA made from Flaveria bidentis and Flaveria brownii with a maximum content of 1 mg/g fresh weight. In leaves of transgenic maize, PPDK molecules produced from the transgene are detected in cold-tolerant homotetramers or in heterotetramers of intermediate cold susceptibility formed with the internal PPDK. A significant improvement in the cold stability of PPDK can be achieved when a suffcient quantity of cold-tolerant subunits is expressed in transgenic maize leaves | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + phosphate | Flaveria bidentis | - |
AMP + phosphoenolpyruvate + diphosphate | - |
? | |
ATP + pyruvate + phosphate | Flaveria brownii | - |
AMP + phosphoenolpyruvate + diphosphate | - |
? | |
ATP + pyruvate + phosphate | Zea mays | loses activity below about 12°C by dissociation of the tetramer, considered as one possible cause of the reduction of the photosynthetic rate of maize at low temperatures | AMP + phosphoenolpyruvate + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flaveria bidentis | - |
- |
- |
Flaveria brownii | - |
- |
- |
Zea mays | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + phosphate | - |
Zea mays | AMP + phosphoenolpyruvate + diphosphate | - |
? | |
ATP + pyruvate + phosphate | - |
Flaveria bidentis | AMP + phosphoenolpyruvate + diphosphate | - |
? | |
ATP + pyruvate + phosphate | - |
Flaveria brownii | AMP + phosphoenolpyruvate + diphosphate | - |
? | |
ATP + pyruvate + phosphate | loses activity below about 12°C by dissociation of the tetramer, considered as one possible cause of the reduction of the photosynthetic rate of maize at low temperatures | Zea mays | AMP + phosphoenolpyruvate + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PPDK | - |
Zea mays |
PPDK | - |
Flaveria bidentis |
PPDK | - |
Flaveria brownii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
12 | - |
loses activity below at about 12°C by dissociation of the tetramer, considered as one possible cause of the reduction of the photosynthetic rate of maize at low temperatures | Zea mays |