Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | product inhibition versus phosphoenolpyruvate is noncompetitive | Entamoeba histolytica | |
phosphate | inhibits rate of ATP synthesis at saturating and equimolar concentrations of phosphoenolpyruvate, AMP and diphosphate. Product inhibition versus phosphoenolpyruvate is uncompetitive | Entamoeba histolytica | |
pyruvate | - |
Entamoeba histolytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + phosphoenolpyruvate + diphosphate | Entamoeba histolytica | synthesis of ATP is not thermodynamically favorable in trophozoites of Entamoeba histolytica | ATP + pyruvate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | - |
recombinant enzyme expressed in Escherichia coli | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + phosphoenolpyruvate + diphosphate | synthesis of ATP is not thermodynamically favorable in trophozoites of Entamoeba histolytica | Entamoeba histolytica | ATP + pyruvate + phosphate | - |
? | |
AMP + phosphoenolpyruvate + diphosphate | ordered steady-state mechanism, uni uni bi bi ping-pong mechanismsequential kinetic mechanism between AMP and pyrophosphate, phosphate is released before ATP | Entamoeba histolytica | ATP + pyruvate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EhPPDH | - |
Entamoeba histolytica |
pyruvate phosphate dikinase | - |
Entamoeba histolytica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
ATP | with phosphoenolpyruvate as varied substrate, Ki(slope) | Entamoeba histolytica | |
0.4 | - |
pyruvate | with diphosphate as varied substrate, Ki(intercept) | Entamoeba histolytica | |
0.45 | - |
ATP | withAMP as varied substrate | Entamoeba histolytica | |
2.3 | - |
pyruvate | with diphosphate as varied substrate, Ki(slope) | Entamoeba histolytica | |
5.1 | - |
ATP | with diphosphate as varied substrate, Ki(slope) | Entamoeba histolytica | |
7.2 | - |
phosphate | with phosphoenolpyruvate as varied substrate | Entamoeba histolytica | |
15 | - |
ATP | with diphosphate as varied substrate, Ki(intercept) | Entamoeba histolytica | |
17 | - |
ATP | with phosphoenolpyruvate as varied substrate, Ki(intercept) | Entamoeba histolytica | |
22.6 | - |
pyruvate | with AMP as varied substrate | Entamoeba histolytica | |
23 | - |
phosphate | with diphosphate as varied substrate, Ki(slope) | Entamoeba histolytica | |
44 | - |
phosphate | with AMP as varied substrate, Ki(slope) | Entamoeba histolytica | |
46 | - |
phosphate | with diphosphate as varied substrate, Ki(intercept) | Entamoeba histolytica | |
88 | - |
phosphate | with AMP as varied substrate, Ki(intercept) | Entamoeba histolytica |