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Literature summary for 2.7.9.1 extracted from

  • Varela-Gomez, M.; Moreno-Sanchez, R.; Pardo, J.P.; Perez-Montfort, R.
    Kinetic mechanism and metabolic role of pyruvate phosphate dikinase from Entamoeba histolytica (2004), J. Biol. Chem., 279, 54124-54130.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ATP product inhibition versus phosphoenolpyruvate is noncompetitive Entamoeba histolytica
phosphate inhibits rate of ATP synthesis at saturating and equimolar concentrations of phosphoenolpyruvate, AMP and diphosphate. Product inhibition versus phosphoenolpyruvate is uncompetitive Entamoeba histolytica
pyruvate
-
Entamoeba histolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
AMP + phosphoenolpyruvate + diphosphate Entamoeba histolytica synthesis of ATP is not thermodynamically favorable in trophozoites of Entamoeba histolytica ATP + pyruvate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica
-
recombinant enzyme expressed in Escherichia coli
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AMP + phosphoenolpyruvate + diphosphate synthesis of ATP is not thermodynamically favorable in trophozoites of Entamoeba histolytica Entamoeba histolytica ATP + pyruvate + phosphate
-
?
AMP + phosphoenolpyruvate + diphosphate ordered steady-state mechanism, uni uni bi bi ping-pong mechanismsequential kinetic mechanism between AMP and pyrophosphate, phosphate is released before ATP Entamoeba histolytica ATP + pyruvate + phosphate
-
?

Synonyms

Synonyms Comment Organism
EhPPDH
-
Entamoeba histolytica
pyruvate phosphate dikinase
-
Entamoeba histolytica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.14
-
ATP with phosphoenolpyruvate as varied substrate, Ki(slope) Entamoeba histolytica
0.4
-
pyruvate with diphosphate as varied substrate, Ki(intercept) Entamoeba histolytica
0.45
-
ATP withAMP as varied substrate Entamoeba histolytica
2.3
-
pyruvate with diphosphate as varied substrate, Ki(slope) Entamoeba histolytica
5.1
-
ATP with diphosphate as varied substrate, Ki(slope) Entamoeba histolytica
7.2
-
phosphate with phosphoenolpyruvate as varied substrate Entamoeba histolytica
15
-
ATP with diphosphate as varied substrate, Ki(intercept) Entamoeba histolytica
17
-
ATP with phosphoenolpyruvate as varied substrate, Ki(intercept) Entamoeba histolytica
22.6
-
pyruvate with AMP as varied substrate Entamoeba histolytica
23
-
phosphate with diphosphate as varied substrate, Ki(slope) Entamoeba histolytica
44
-
phosphate with AMP as varied substrate, Ki(slope) Entamoeba histolytica
46
-
phosphate with diphosphate as varied substrate, Ki(intercept) Entamoeba histolytica
88
-
phosphate with AMP as varied substrate, Ki(intercept) Entamoeba histolytica