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Literature summary for 2.7.9.1 extracted from

  • Jenkins, C.L.D.; Hatch, M.D.
    Properties and reaction mechanism of C4 leaf pyruvate,Pi dikinase (1985), Arch. Biochem. Biophys., 239, 53-62.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
AMP non competitive with respect to ATP Zea mays
diphosphate non competitive to phosphate Zea mays
phosphoenolpyruvate competitive to pyruvate Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
ATP pH 7.4, 25ºC, phosphoenolpyruvate formation Zea mays
0.046
-
phosphoenolpyruvate pH 7.4, 25ºC, pyruvate formation Zea mays
0.082
-
ATP pH 8.1, 25ºC, phosphoenolpyruvate formation Zea mays
0.082
-
pyruvate pH 8.1, 25ºC, phosphoenolpyruvate formation Zea mays
0.092
-
pyruvate pH 7.4, 25ºC, phosphoenolpyruvate formation Zea mays
0.16
-
phosphoenolpyruvate pH 7.4, 25ºC, pyruvate formation Zea mays
0.38
-
phosphate pH 8.1, 25ºC, phosphoenolpyruvate formation Zea mays
0.56
-
phosphate pH 8.1, 25ºC, phosphoenolpyruvate formation Zea mays

Metals/Ions

Metals/Ions Comment Organism Structure
K+ more than 20fold activation pH 7.4 and at pH 8.1 Zea mays
Na+ slight activation of the phosphoenol pyruvate formation Zea mays
NH4+ more than 20fold activation at pH 7.4 and at pH 8.1 Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays
-
var. Dekalb XL 81
-

Purification (Commentary)

Purification (Comment) Organism
-
Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyruvate + phosphate
-
Zea mays AMP + phosphoenolpyruvate + diphosphate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9
-
pyruvate formation Zea mays
8.2
-
phosphoenolpyruvate formation Zea mays

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.13
-
AMP pH 8.1, 25ºC, phosphoenolpyruvate formation Zea mays
0.15
-
phosphoenolpyruvate pH 8.1, 25ºC, phosphoenolpyruvate formation Zea mays
0.32
-
diphosphate pH 8.1, 25ºC, phosphoenolpyruvate formation Zea mays