Crystallization (Comment) | Organism |
---|---|
method description | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
ATP | pH 7.5, 22ºC, phosphoenolpyruvate formation | Zea mays | |
0.04 | - |
diphosphate | pH 7.5, 22ºC, pyruvate formation | Zea mays | |
0.14 | - |
phosphoenolpyruvate | pH 7.5, 22ºC, pyruvate formation | Zea mays | |
0.25 | - |
pyruvate | pH 7.5, 22ºC, phosphoenolpyruvate formation | Zea mays | |
1.5 | - |
phosphate | pH 7.5, 22ºC, phosphoenolpyruvate formation | Zea mays |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | free Mg2+ is required to stabilize the oligomeric structure of the enzyme | Zea mays | |
NH4+ | at saturated concentration of NH4Cl pyruvate formation activity is increased 20fold | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
94000 | - |
4 * 94000, SDS-PAGE | Zea mays |
370000 | - |
gel filtration on Sepharose 6B | Zea mays |
387000 | - |
sedimentation analysis | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Zea mays | involved in C4 dicarboxylic acid pathway in plant | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Zea mays | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + phosphate | - |
Zea mays | AMP + phosphoenolpyruvate + diphosphate | - |
? | |
additional information | involved in C4 dicarboxylic acid pathway in plant | Zea mays | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 94000, SDS-PAGE | Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Zea mays |