Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | phosphoenolpyruvate formation, competitive to ATP | Komagataeibacter xylinus | |
ATP | pyruvate formation, competitive to AMP | Komagataeibacter xylinus | |
p-hydroxymercuribenzoate | - |
Komagataeibacter xylinus | |
potassium fluoride | inhibits reaction in both directions at 50 mM | Komagataeibacter xylinus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
AMP | pH 6.5, 30ºC, pyruvate formation | Komagataeibacter xylinus | |
0.06 | - |
diphosphate | pH 6.5, 30ºC, pyruvate formation | Komagataeibacter xylinus | |
0.1 | - |
phosphoenolpyruvate | pH 6.5, 30ºC, pyruvate formation | Komagataeibacter xylinus | |
0.2 | - |
pyruvate | pH 8.2, 30ºC, phosphoenolpyruvate formation | Komagataeibacter xylinus | |
0.4 | - |
ATP | pH 8.2, 30ºC, phosphoenolpyruvate formation | Komagataeibacter xylinus | |
0.8 | - |
phosphate | pH 8.2, 30ºC, phosphoenolpyruvate formation | Komagataeibacter xylinus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Komagataeibacter xylinus | |
Mn2+ | cannot replace Mg2+ in either the forward or the reverse reaction | Komagataeibacter xylinus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + phosphate | Komagataeibacter xylinus | enzyme activity has implication in the regulation of gluconeogenesis and carbohydrate oxidation | AMP + phosphoenolpyruvate + diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Komagataeibacter xylinus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, using ammonium sulfate precipitation and chromatography on DEAE-cellulose | Komagataeibacter xylinus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.88 | - |
- |
Komagataeibacter xylinus |
Storage Stability | Organism |
---|---|
retains 85% of activity after two weeks at room temperature | Komagataeibacter xylinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + arsenate | the rate of phosphoenolpyruvate formation in the presence of arsenate is 65% lower than that obtained with phosphate | Komagataeibacter xylinus | ? | - |
? | |
ATP + pyruvate + phosphate | GTP, CTP, ITP or TTP cannot replace ATP in the reaction with pyruvate | Komagataeibacter xylinus | AMP + phosphoenolpyruvate + diphosphate | GDP, CMP and ADP cannot replace AMP in the reverse reaction | r | |
ATP + pyruvate + phosphate | enzyme activity has implication in the regulation of gluconeogenesis and carbohydrate oxidation | Komagataeibacter xylinus | AMP + phosphoenolpyruvate + diphosphate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Komagataeibacter xylinus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
pyruvate formation | Komagataeibacter xylinus |
8.2 | - |
phosphoenolpyruvate formation | Komagataeibacter xylinus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
AMP | pH 8.2, 30ºC, phosphoenolpyruvate formation | Komagataeibacter xylinus | |
0.22 | - |
ATP | pH 6.5, 30ºC, pyruvate formation | Komagataeibacter xylinus |