Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
[Clostridium] symbiosum | |
CTP | total inhibition of the forward reaction at 1 mM | [Clostridium] symbiosum | |
ITP | total inhibition of the forward reaction at 1 mM | [Clostridium] symbiosum | |
additional information | no tested 5'-nucleoside monophosphate inhibits the reaction with AMP | [Clostridium] symbiosum | |
oxalate | - |
[Clostridium] symbiosum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0035 | - |
AMP | - |
[Clostridium] symbiosum | |
0.08 | - |
pyruvate | pH 6.8, 25ºC, pyruvate formation | [Clostridium] symbiosum | |
0.1 | - |
ATP | pH 6.8, 25ºC, pyruvate formation | [Clostridium] symbiosum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
94000 | - |
x * 94000, SDS-PAGE | [Clostridium] symbiosum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | [Clostridium] symbiosum | functions in glycolytic pathway | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Clostridium] symbiosum | - |
ATCC 14940 | - |
Purification (Comment) | Organism |
---|---|
partial, using ammonium sulfate precipitation, and chromatography on DEAE-cellulose and Bio-Gel | [Clostridium] symbiosum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
[Clostridium] symbiosum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + phosphate | specificity is strictly restricted to adenine nucleotides | [Clostridium] symbiosum | AMP + phosphoenolpyruvate + diphosphate | - |
? | |
additional information | functions in glycolytic pathway | [Clostridium] symbiosum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 94000, SDS-PAGE | [Clostridium] symbiosum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | [Clostridium] symbiosum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
reverse reaction, pyruvate formation | [Clostridium] symbiosum |
7.2 | 8 | forward reaction, phosphoenolpyruvate formation | [Clostridium] symbiosum |