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Literature summary for 2.7.8.23 extracted from
- Cloning, overexpression and mechanistic studies of carboxyphosphoenolpyruvate mutase from Streptomyces hygroscopicus (1992), Eur. J. Biochem., 209, 735-743.
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | formation of one of the two C-P bonds in the tripeptide antibiotic bialaphos, a potent herbicide isolated from Streptomyces hygroscopicus SF1293, enzyme is produced in large amounts by high producer strain HP5-29, enzyme may contribute to improvement of bialphos production | Streptomyces hygroscopicus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cloned from a subgenomic library cloned by colony hybridisation in Escherichia coli AG1, subcloned into vector pET11a and overexpressed in Escherichia coli BL21(DE3) | Streptomyces hygroscopicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0022 | - |
carboxyphosphinopyruvate | pH 6.5, 30°C | Streptomyces hygroscopicus |  |
| 0.27 | - |
carboxyphosphoenolpyruvate | pH 6.5, 30°C | Streptomyces hygroscopicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | reaction shows metal dependence | Streptomyces hygroscopicus | |
| Mn2+ | increases activity, about 10times more active compared with the activity in presence of Mg2+ | Streptomyces hygroscopicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxyphosphonoenolpyruvate | Streptomyces hygroscopicus | biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos | (hydroxyphosphinyl)pyruvate + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| no activity in Streptomyces hygroscopicus | - |
- |
- |
| Streptomyces hygroscopicus | - |
ATCC 21705 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces hygroscopicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
specific activity 37.6 U/mg x 1000 | Streptomyces hygroscopicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxyphosphinopyruvate | - |
Streptomyces hygroscopicus | (hydroxyphosphinyl)pyruvate + CO2 | - |
? | |
| carboxyphosphoenolpyruvate | - |
Streptomyces hygroscopicus | ? | - |
? | |
| carboxyphosphonoenolpyruvate | - |
Streptomyces hygroscopicus | (hydroxyphosphinyl)pyruvate + CO2 | - |
? | |
| carboxyphosphonoenolpyruvate | biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos | Streptomyces hygroscopicus | (hydroxyphosphinyl)pyruvate + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| carboxyphosphoenolpyruvate mutase | - |
Streptomyces hygroscopicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00076 | - |
carboxyphosphinopyruvate | pH 7.5, 30°C | Streptomyces hygroscopicus | |
| 0.02 | - |
carboxyphosphoenolpyruvate | pH 7.5, 30°C | Streptomyces hygroscopicus | |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | stable in this range | Streptomyces hygroscopicus |
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