Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Dolichyl-phosphoryl-mannose | stimulates | Bos taurus |
General Stability | Organism |
---|---|
purified enzyme is resistant to digestion with either endo-beta-N-acetylglucosaminidase H or F | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Diumycin | - |
Bos taurus | |
dolichyl phosphate | at high concentrations | Bos taurus | |
Hg2+ | partially reversed by dithiothreitol | Bos taurus | |
iodoacetamide | - |
Bos taurus | |
PCMB | - |
Bos taurus | |
tunicamycin | - |
Bos taurus | |
UDP | - |
Bos taurus | |
UDP-glucose | - |
Bos taurus | |
UDP-hexanolamine | - |
Bos taurus | |
UDP-xylose | - |
Bos taurus | |
UMP | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
UDP-GlcNAc | pH 7.6, 37°C | Bos taurus | |
0.016 | - |
dolichyl phosphate | pH 7.6, 37°C | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Bos taurus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | stimulates | Bos taurus | |
Mn2+ | stimulates | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
330000 | 360000 | large molecular mass of the enzyme can be due to aggregation of the hydrophobic, membrane-derived enzyme rather than resulting from subunit interaction, gel filtration | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + dolichyl phosphate | Bos taurus | enzyme initiates the dolichol cycle for the biosynthesis of asparagine-linked glycoproteins | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | the enzyme is not a glycoprotein of the asparagine-linked type | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mammary gland | lactating | Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
4°C, solubilized enzyme loses most of its activity within 12 h | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate | - |
Bos taurus | UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol | - |
? | |
UDP-N-acetyl-D-glucosamine + dolichyl phosphate | enzyme initiates the dolichol cycle for the biosynthesis of asparagine-linked glycoproteins | Bos taurus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | results indicate that either 70000 MW band in SDS-PAGE is a precursor form of the enzyme or this polypeptide, representing the native enzyme or its subunit, is proteolyzed to smaller, enzymatically active peptides of 50000 Da and 46000 Da during purification | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 7.6 | - |
Bos taurus |