Any feedback?
Literature summary for 2.7.8.15 extracted from
- Properties of a soluble polyprenyl phosphate. UDP-D-N-acetylglucosamine N-acetylglucosamine-1-phosphate transferase (1980), J. Biol. Chem., 255, 8174-8178.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Diumycin | - |
Acanthamoeba castellanii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0005 | - |
UDP-GlcNAc | pH 7.4, 30°C | Acanthamoeba castellanii |  |
| 0.0045 | - |
dolichyl phosphate | pH 7.4, 30°C | Acanthamoeba castellanii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | divalent cation required for dialyzed enzyme, maximal activity in presence of 8-10 mM | Acanthamoeba castellanii | |
| Mn2+ | divalent cation required for dialyzed enzyme, Mg2+ or Mn2+ | Acanthamoeba castellanii | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acanthamoeba castellanii | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Acanthamoeba castellanii | - |
| cyst | - |
Acanthamoeba castellanii | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C or -70°C, stable for 2 weeks | Acanthamoeba castellanii |
| 4°C, completely stable for 9-10 days. No significant loss of activity after 63 days in presence of NaN3 | Acanthamoeba castellanii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate | - |
Acanthamoeba castellanii | UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Acanthamoeba castellanii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 40 | 0°C: no activity, 15°C: 11% of maximal activity, 25°C: 73% of maximal activity, 35°C: 96% of maximal activity, 40°C: 57% of maximal activity | Acanthamoeba castellanii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
1 h, stable | Acanthamoeba castellanii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 7.8 | - |
Acanthamoeba castellanii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.7 | pH 6.0: no activity, pH 7.0: 85% of maximal activity, pH 8.7: 64% of maximal activity | Acanthamoeba castellanii |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
