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Literature summary for 2.7.8.15 extracted from

  • Sharma, C.B.; Lehle, L.; Tanner, W.
    Solubilization and characterization of the initial enzymes of the dolichol pathway from yeast (1982), Eur. J. Biochem., 126, 319-325.
    View publication on PubMed
Search for more literature for 2.7.8.15

General Stability

General Stability Organism
a 1:4 dilution leads to a 65% loss of activity Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
tunicamycin 1 mM, complete inhibition Saccharomyces cerevisiae
UDP 1 mM, 55% inhibition Saccharomyces cerevisiae
UMP 1 mM, 45% inhibition Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 dolichyl phosphate pH 7.5 Saccharomyces cerevisiae
0.015
-
 UDP-GlcNAc pH 7.5 Saccharomyces cerevisiae
0.18
-
 dolichyl phosphate pH 7.5 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Saccharomyces cerevisiae 16020
-
microsome
-
 Saccharomyces cerevisiae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ divalent metal required, Ca2+ is less effective than Mg2+ Saccharomyces cerevisiae
Mg2+ divalent metal required, optimal activity in presence of Mg2+ Saccharomyces cerevisiae
Mn2+ divalent metal required, Mn2+ is less effective than Mg2+ Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-glucosamine + dolichyl phosphate Saccharomyces cerevisiae first enzyme of dolichol pathway ?
-
 ?
UDP-N-acetyl-D-glucosamine + dolichyl phosphate Saccharomyces cerevisiae X2180-1A first enzyme of dolichol pathway ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 X2180-1A
-
Saccharomyces cerevisiae X2180-1A
-
 X2180-1A
-

Storage Stability

Storage Stability Organism
-20°C, solubilized enzyme loses 96% of its activity after 6 days, only 20% loss of activity after 6 days in presence of 0.07 mM UDP-GlcNAc Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
 Saccharomyces cerevisiae UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
 ?
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
 Saccharomyces cerevisiae X2180-1A UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
 ?
UDP-N-acetyl-D-glucosamine + dolichyl phosphate first enzyme of dolichol pathway Saccharomyces cerevisiae ?
-
 ?
UDP-N-acetyl-D-glucosamine + dolichyl phosphate first enzyme of dolichol pathway Saccharomyces cerevisiae X2180-1A ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 pH 6.5: about 60% of maximal activity, pH 8.5: about 35% of maximal activity Saccharomyces cerevisiae