Protein Variants | Comment | Organism |
---|---|---|
additional information | an artificial gene construct for a protein homologous to phosphatidylinositol phosphate synthase of Mycobacterium tuberculosis and archaetidylinositol phosphate synthase of Methanothermobacter thermautotrophicus is cloned in Escherichia coli and introduced into Homo sapiens cells. The recombinant cells do not show phosphatidylinositol phosphate synthase or archaetidylinositol phosphate synthase activities. Phosphatidylinositol synthase and archaetidylinositol synthase activities are determined, 7 pmol/h/mg and 2 pmol/h/mg, respectively | Homo sapiens |
additional information | an artificial gene construct for a protein homologous to phosphatidylinositol phosphate synthase of Mycobacterium tuberculosis and archaetidylinositol phosphate synthase of Methanothermobacter thermautotrophicus is cloned in Escherichia coli and introduced into Saccharomyces cerevisiae cells. The recombinant cells do not show phosphatidylinositol phosphate synthase or archaetidylinositol phosphate synthase activities. Phosphatidylinositol synthase and archaetidylinositol synthase activities are determined, 107 pmol/h/mg and 7 pmol/h/mg, respectively | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Homo sapiens | |
Mn2+ | required | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP-diacylglycerol + myo-inositol | Homo sapiens | - |
CMP + 1-phosphatidyl-1D-myo-inositol | - |
? | |
CDP-diacylglycerol + myo-inositol | Saccharomyces cerevisiae | - |
CMP + 1-phosphatidyl-1D-myo-inositol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Saccharomyces cerevisiae | P70500 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP-diacylglycerol + myo-inositol | - |
Homo sapiens | CMP + 1-phosphatidyl-1D-myo-inositol | - |
? | |
CDP-diacylglycerol + myo-inositol | - |
Saccharomyces cerevisiae | CMP + 1-phosphatidyl-1D-myo-inositol | - |
? | |
CDP-diacylglycerol + myo-inositol | specificity of PI synthase for the substrates, no activity with 1L-myo-inositol 1-phosphate | Homo sapiens | CMP + 1-phosphatidyl-1D-myo-inositol | - |
? | |
CDP-diacylglycerol + myo-inositol | specificity of PI synthase for the substrates, no activity with 1L-myo-inositol 1-phosphate | Saccharomyces cerevisiae | CMP + 1-phosphatidyl-1D-myo-inositol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phosphatidylinositol synthase | - |
Homo sapiens |
phosphatidylinositol synthase | - |
Saccharomyces cerevisiae |
PI synthase | - |
Homo sapiens |
PI synthase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
8 | - |
assay at | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
metabolism | in eukarya, phosphatidylinositol (PI) is only biosynthesized from CDP-diacylglycerol (CDP-DAG) and inositol | Homo sapiens |
metabolism | in eukarya, phosphatidylinositol (PI) is only biosynthesized from CDP-diacylglycerol (CDP-DAG) and inositol | Saccharomyces cerevisiae |